@article {16598,
	title = {Development of a real-time PCR detection method for a FCGR2A polymorphism in the LightCycler and application in the heparin-induced thrombocytopenia syndrome},
	journal = {Clin.Biochem.},
	volume = {42},
	year = {2009},
	note = {DA - 20091012
IS - 1873-2933 (Electronic)
IS - 0009-9120 (Linking)
LA - eng
PT - Journal Article
RN - 0 (FCGR2A protein, human)
RN - 0 (Receptors, IgG)
RN - 9005-49-6 (Heparin)
RN - 9007-49-2 (DNA)
SB - IM},
	month = {2009/11//},
	pages = {1685 - 1693},
	abstract = {OBJECTIVE: The FcgammaRIIa receptor is responsible for the activation of platelets by antibodies in heparin-induced thrombocytopenia (HIT). The c.497G>A polymorphism in the corresponding FCG2RA gene (H131R) has been implicated in the HIT syndrome and we aimed at its rapid and reliable determination. DESIGN AND METHODS: We designed a novel asymmetric real-time PCR method in the LightCycler that uses two hybridization probes and is followed by melting curve analysis. Seventy-one post-cardiac-surgery HIT Greek patients well ascertained by clinical data, immunological and functional tests (PAT, CD62P-selectin and microparticle flow cytometric detection) were studied, along with a clinically relevant group of 49 thrombocytopenic control patients and 119 healthy subjects. RESULTS: The developed method has excellent analytical characteristics (linear and efficient amplification, precision), has wide DeltaT(m) between the two alleles H and R (11.53 degrees C), and is in 100\% concordance with validated controls and another commonly used screening method. The RR percentage increased from 10\% in the control populations to 24\% in the HIT patient group. CONCLUSION: The described method is technically simple, robust, fast, and accurate. A statistically significant difference was found in the comparison between the groups of HIT patients and healthy subjects [RR vs. RH+ HH, chi(2) test, p=0.01, OR (95\% C.I.) 2.81 (1.21-4.68)]. The RR frequency in the Greek population was found to be the lowest among Caucasians},
	keywords = {Adverse effects, analysis, Base Sequence, chemically induced, DNA, genetics, Genotype, Greece, Heparin, Humans, methods, Molecular Sequence Data, polymerase chain reaction, Polymorphism,Genetic, Receptors,IgG, Thrombocytopenia},
	url = {PM:19664613},
	author = {Kroupis, C. and Theodorou, M. and Kounavi,M. and Oliveira, S.C. and Iliopoulou,E. and Mavri-Vavayanni,M. and Melissari,E.N. and Degiannis, D.}
}
