@article {16600,
	title = {Asymmetric real-time PCR detection of BRCA1 5382insC mutation by melting curve analysis in the LightCycler},
	journal = {Clin.Chim.Acta},
	volume = {390},
	year = {2008},
	note = {DA - 20080303
IS - 0009-8981 (Print)
IS - 0009-8981 (Linking)
LA - eng
PT - Journal Article
RN - 0 (BRCA1 Protein)
RN - 0 (DNA Primers)
SB - IM},
	month = {2008/04//},
	pages = {141 - 144},
	abstract = {BACKGROUND: 5382insC BRCA1 frameshift mutation is a common founder mutation for many populations worldwide and a high-risk allele for the development of hereditary breast and/or ovarian cancer. Our goal was to develop a novel, reliable and rapid method for its detection. METHODS: We developed an asymmetric real-time PCR method with hybridization probes in the LightCycler. Genotyping was performed by melting curve analysis. RESULTS AND CONCLUSIONS: The developed method was in concordance with reference methods when tested in 85 peripheral blood and 107 tumor DNA samples from Greek breast and/or ovarian cancer patients. The described method proved to be simple, cost-effective, easy to perform and rapid enough for routine use as a screening method in high-risk families and especially in the Greek, Slavic and Jewish populations where 5382insC mutation is the most common BRCA1 mutation},
	keywords = {analysis, Base Sequence, Blood, BRCA1 Protein, Breast Neoplasms, DNA Primers, Female, genetics, Greece, Humans, methods, Mutation, Ovarian Neoplasms, polymerase chain reaction},
	url = {PM:18242166},
	author = {Kroupis, C. and Christopoulos,K. and Devetzoglou,M. and Tsiagas,I. and Lianidou,E.S.}
}
