@article {16622,
	title = {ATP-dependent regulation of sodium-calcium exchange in Chinese hamster ovary cells transfected with the bovine cardiac sodium-calcium exchanger},
	journal = {J.Biol.Chem.},
	volume = {270},
	year = {1995},
	note = {DA - 19950523
IS - 0021-9258 (Print)
IS - 0021-9258 (Linking)
LA - eng
PT - Journal Article
PT - Research Support, U.S. Gov{\textquoteright}t, P.H.S
RN - 0 (Actins)
RN - 0 (Carrier Proteins)
RN - 0 (Sodium-Calcium Exchanger)
RN - 22144-77-0 (Cytochalasin D)
RN - 8L70Q75FXE (Adenosine Triphosphate)
RN - 9NEZ333N27 (Sodium)
RN - SY7Q814VUP (Calcium)
SB - IM},
	month = {1995/04/21/},
	pages = {9137 - 9146},
	abstract = {Chinese hamster ovary cells expressing the bovine cardiac Na/Ca exchanger were treated with ouabain to increase [Na+]i and stimulate Ca2+ influx by Na/Ca exchange. Depletion of cellular ATP inhibited 45Ca uptake by 40\% or more and reduced the half-maximal Na+ concentration for inhibition of 45Ca uptake from 90 to 55 mM. ATP depletion also reduced the rate of rise in [Ca2+]i when [Na+]o was reduced and inhibited the decline in [Ca2+]i when high [Na+]o was restored. The effects of ATP depletion were either absent or reduced in cells expressing a mutant exchanger missing most of the cytosolic hydrophilic domain. We were unable to detect a phosphorylated form of the exchanger in immunoprecipitates from 32P-labeled cells. ATP depletion caused a breakdown in the actin cytoskeleton of the cells. Treatment of the cells with cytochalasin D mimicked the effects of ATP depletion on the [Na+] inhibition profile for 45Ca uptake. Thus, ATP depletion inhibits both the Ca2+ influx and Ca2+ efflux modes of Na/Ca exchange, and may alter the competitive interactions of extracellular Na+ and Ca2+ with the transporter. The latter effect appears to be related to changes in the actin cytoskeleton},
	keywords = {Actins, Adenosine Triphosphate, Animals, calcium, Carrier Proteins, Cattle, Cho Cells, Cricetinae, Cytochalasin D, Hydrogen-Ion Concentration, metabolism, Myocardium, pharmacology, physiology, sodium, Sodium-Calcium Exchanger, Transfection},
	url = {PM:7721828},
	author = {Condrescu,M. and Gardner,J.P. and Chernaya,G. and Aceto,J.F. and Kroupis, C. and Reeves,J.P.}
}
