@article {16624,
	title = {Stable expression of the cardiac sodium-calcium exchanger in CHO cells},
	journal = {Am.J.Physiol},
	volume = {264},
	year = {1993},
	note = {DA - 19930517
IS - 0002-9513 (Print)
IS - 0002-9513 (Linking)
LA - eng
PT - Journal Article
PT - Research Support, U.S. Gov{\textquoteright}t, P.H.S
RN - 0 (Calcium Radioisotopes)
RN - 0 (Carrier Proteins)
RN - 0 (Sodium-Calcium Exchanger)
RN - 5ACL011P69 (Ouabain)
RN - 9NEZ333N27 (Sodium)
RN - AE28F7PNPL (Methionine)
RN - RWP5GA015D (Potassium)
RN - SY7Q814VUP (Calcium)
SB - IM},
	month = {1993/04//},
	pages = {C1066 - C1074},
	abstract = {A line of Chinese hamster ovary (CHO) cells called CK1.4 was produced by transfection with the gene for the bovine cardiac Na(+)-Ca2+ exchanger. CK1.4 cells stably expressed substantial exchange activity and exchanger protein as shown by immunoprecipitation. Exchange activity was quantified as 45Ca2+ influx that depended on both increasing intracellular Na+ and lowering the concentration of external Na+. Replacing external Na+ with K+ slightly increased 45Ca2+ uptake by CK1.4 cells with basal Na+ and greatly increased 45Ca2+ uptake by Na(+)-loaded cells. Neither exchange activity nor exchanger protein was detected in the nontransfected parental line. By contrast to CK1.4 cells, replacing external Na+ with K+ decreased 45Ca2+ uptake in the nontransfected cells whether or not they were Na+ loaded. Changes in cytosolic free Ca2+ determined with fura-2 were consistent with the 45Ca2+ uptake data. Analysis of poly(A)(+)-RNA by Northern blot confirmed that CK1.4 cells, but not the parental line, expressed the exchanger. Expression of the exchanger was also observed in aortic myocytes and a renal epithelial cell line (LLC-MK2) but not in other lines of renal epithelial cells (MDCK, LLC-PK1) or human dermal fibroblasts. The cardiac exchanger produced substantial 45Ca2+ efflux from CK1.4 cells in response to hormone-evoked release of stored Ca2+. CK1.4 cells are an attractive model for studies of the regulation of the cardiac exchanger because they stably express sufficient exchanger for biochemical and immunological analysis},
	keywords = {analysis, Animals, Aorta, Biological Transport,Active, calcium, Calcium Radioisotopes, Carrier Proteins, Cattle, Cho Cells, Cricetinae, Cytosol, genetics, Heart, Intracellular Fluid, isolation \& purification, Kidney, Kinetics, metabolism, Methionine, Muscle,Smooth,Vascular, Ouabain, pharmacology, physiology, potassium, sodium, Sodium-Calcium Exchanger, Transfection},
	url = {PM:8476012},
	author = {Pijuan,V. and Zhuang,Y. and Smith,L. and Kroupis, C. and Condrescu,M. and Aceto,J.F. and Reeves,J.P. and Smith,J.B.}
}
