<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kroupis, C.</style></author><author><style face="normal" font="default" size="100%">Theodorou, M.</style></author><author><style face="normal" font="default" size="100%">Kounavi,M.</style></author><author><style face="normal" font="default" size="100%">Oliveira, S.C.</style></author><author><style face="normal" font="default" size="100%">Iliopoulou,E.</style></author><author><style face="normal" font="default" size="100%">Mavri-Vavayanni,M.</style></author><author><style face="normal" font="default" size="100%">Melissari,E.N.</style></author><author><style face="normal" font="default" size="100%">Degiannis, D.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Development of a real-time PCR detection method for a FCGR2A polymorphism in the LightCycler and application in the heparin-induced thrombocytopenia syndrome</style></title><secondary-title><style face="normal" font="default" size="100%">Clin.Biochem.</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Adverse effects</style></keyword><keyword><style  face="normal" font="default" size="100%">analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Base Sequence</style></keyword><keyword><style  face="normal" font="default" size="100%">chemically induced</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Genotype</style></keyword><keyword><style  face="normal" font="default" size="100%">Greece</style></keyword><keyword><style  face="normal" font="default" size="100%">Heparin</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Sequence Data</style></keyword><keyword><style  face="normal" font="default" size="100%">polymerase chain reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymorphism,Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Receptors,IgG</style></keyword><keyword><style  face="normal" font="default" size="100%">Thrombocytopenia</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2009/11//</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">PM:19664613</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">42</style></volume><pages><style face="normal" font="default" size="100%">1685 - 1693</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">OBJECTIVE: The FcgammaRIIa receptor is responsible for the activation of platelets by antibodies in heparin-induced thrombocytopenia (HIT). The c.497G&gt;A polymorphism in the corresponding FCG2RA gene (H131R) has been implicated in the HIT syndrome and we aimed at its rapid and reliable determination. DESIGN AND METHODS: We designed a novel asymmetric real-time PCR method in the LightCycler that uses two hybridization probes and is followed by melting curve analysis. Seventy-one post-cardiac-surgery HIT Greek patients well ascertained by clinical data, immunological and functional tests (PAT, CD62P-selectin and microparticle flow cytometric detection) were studied, along with a clinically relevant group of 49 thrombocytopenic control patients and 119 healthy subjects. RESULTS: The developed method has excellent analytical characteristics (linear and efficient amplification, precision), has wide DeltaT(m) between the two alleles H and R (11.53 degrees C), and is in 100% concordance with validated controls and another commonly used screening method. The RR percentage increased from 10% in the control populations to 24% in the HIT patient group. CONCLUSION: The described method is technically simple, robust, fast, and accurate. A statistically significant difference was found in the comparison between the groups of HIT patients and healthy subjects [RR vs. RH+ HH, chi(2) test, p=0.01, OR (95% C.I.) 2.81 (1.21-4.68)]. The RR frequency in the Greek population was found to be the lowest among Caucasians</style></abstract><issue><style face="normal" font="default" size="100%">16-17</style></issue><notes><style face="normal" font="default" size="100%">DA - 20091012IS - 1873-2933 (Electronic)IS - 0009-9120 (Linking)LA - engPT - Journal ArticleRN - 0 (FCGR2A protein, human)RN - 0 (Receptors, IgG)RN - 9005-49-6 (Heparin)RN - 9007-49-2 (DNA)SB - IM</style></notes></record></records></xml>