<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kroupis, C.</style></author><author><style face="normal" font="default" size="100%">Christopoulos,K.</style></author><author><style face="normal" font="default" size="100%">Devetzoglou,M.</style></author><author><style face="normal" font="default" size="100%">Tsiagas,I.</style></author><author><style face="normal" font="default" size="100%">Lianidou,E.S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Asymmetric real-time PCR detection of BRCA1 5382insC mutation by melting curve analysis in the LightCycler</style></title><secondary-title><style face="normal" font="default" size="100%">Clin.Chim.Acta</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Base Sequence</style></keyword><keyword><style  face="normal" font="default" size="100%">Blood</style></keyword><keyword><style  face="normal" font="default" size="100%">BRCA1 Protein</style></keyword><keyword><style  face="normal" font="default" size="100%">Breast Neoplasms</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA Primers</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Greece</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Mutation</style></keyword><keyword><style  face="normal" font="default" size="100%">Ovarian Neoplasms</style></keyword><keyword><style  face="normal" font="default" size="100%">polymerase chain reaction</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2008/04//</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">PM:18242166</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">390</style></volume><pages><style face="normal" font="default" size="100%">141 - 144</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">BACKGROUND: 5382insC BRCA1 frameshift mutation is a common founder mutation for many populations worldwide and a high-risk allele for the development of hereditary breast and/or ovarian cancer. Our goal was to develop a novel, reliable and rapid method for its detection. METHODS: We developed an asymmetric real-time PCR method with hybridization probes in the LightCycler. Genotyping was performed by melting curve analysis. RESULTS AND CONCLUSIONS: The developed method was in concordance with reference methods when tested in 85 peripheral blood and 107 tumor DNA samples from Greek breast and/or ovarian cancer patients. The described method proved to be simple, cost-effective, easy to perform and rapid enough for routine use as a screening method in high-risk families and especially in the Greek, Slavic and Jewish populations where 5382insC mutation is the most common BRCA1 mutation</style></abstract><issue><style face="normal" font="default" size="100%">1-2</style></issue><notes><style face="normal" font="default" size="100%">DA - 20080303IS - 0009-8981 (Print)IS - 0009-8981 (Linking)LA - engPT - Journal ArticleRN - 0 (BRCA1 Protein)RN - 0 (DNA Primers)SB - IM</style></notes></record></records></xml>