<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">McGinnis,K.</style></author><author><style face="normal" font="default" size="100%">Kroupis, C.</style></author><author><style face="normal" font="default" size="100%">Wilson,D.B.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Dimerization of Thermomonospora fusca beta-1,4-endoglucanase E2</style></title><secondary-title><style face="normal" font="default" size="100%">Biochemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Actinomycetales</style></keyword><keyword><style  face="normal" font="default" size="100%">Blotting,Western</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulase</style></keyword><keyword><style  face="normal" font="default" size="100%">chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Cross-Linking Reagents</style></keyword><keyword><style  face="normal" font="default" size="100%">Disulfides</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrophoresis,Polyacrylamide Gel</style></keyword><keyword><style  face="normal" font="default" size="100%">Enzyme Stability</style></keyword><keyword><style  face="normal" font="default" size="100%">enzymology</style></keyword><keyword><style  face="normal" font="default" size="100%">Glutaral</style></keyword><keyword><style  face="normal" font="default" size="100%">Hot Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">Hydrogen-Ion Concentration</style></keyword><keyword><style  face="normal" font="default" size="100%">metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Weight</style></keyword><keyword><style  face="normal" font="default" size="100%">Mutation</style></keyword><keyword><style  face="normal" font="default" size="100%">Oxidation-Reduction</style></keyword><keyword><style  face="normal" font="default" size="100%">Ultracentrifugation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1993</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1993/08/17/</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">PM:8347613</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">32</style></volume><pages><style face="normal" font="default" size="100%">8146 - 8150</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Unboiled Thermomonospora fusca endoglucanase E2 electrophoresed on SDS-polyacrylamide gels migrated in the range of 80-90 kDa, but when boiled it migrated in the 40-42-kDa range. Sedimentation equilibrium centrifugation as well as chemical cross-linking experiments confirmed that E2 is a dimer. The dimer was reversibly dissociated at low pH. The E2 dimer was stable up to 70 degrees C, but began to dissociate at this temperature after a 30-60-min incubation. A nondimerizing mutant was obtained using region-specific chemical mutagenesis. DNA sequencing of this mutant revealed a single base change that substituted Gly for Glu-263. Chemical modification of carboxylic acid residues in E2 disrupted the dimer interaction</style></abstract><issue><style face="normal" font="default" size="100%">32</style></issue><notes><style face="normal" font="default" size="100%">DA - 19930916IS - 0006-2960 (Print)IS - 0006-2960 (Linking)LA - engPT - Journal ArticlePT - Research Support, U.S. Gov't, Non-P.H.SRN - 0 (Cross-Linking Reagents)RN - 0 (Disulfides)RN - EC 3.2.1.4 (Cellulase)RN - T3C89M417N (Glutaral)SB - IM</style></notes></record></records></xml>