<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pijuan,V.</style></author><author><style face="normal" font="default" size="100%">Zhuang,Y.</style></author><author><style face="normal" font="default" size="100%">Smith,L.</style></author><author><style face="normal" font="default" size="100%">Kroupis, C.</style></author><author><style face="normal" font="default" size="100%">Condrescu,M.</style></author><author><style face="normal" font="default" size="100%">Aceto,J.F.</style></author><author><style face="normal" font="default" size="100%">Reeves,J.P.</style></author><author><style face="normal" font="default" size="100%">Smith,J.B.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Stable expression of the cardiac sodium-calcium exchanger in CHO cells</style></title><secondary-title><style face="normal" font="default" size="100%">Am.J.Physiol</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Aorta</style></keyword><keyword><style  face="normal" font="default" size="100%">Biological Transport,Active</style></keyword><keyword><style  face="normal" font="default" size="100%">calcium</style></keyword><keyword><style  face="normal" font="default" size="100%">Calcium Radioisotopes</style></keyword><keyword><style  face="normal" font="default" size="100%">Carrier Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Cattle</style></keyword><keyword><style  face="normal" font="default" size="100%">Cho Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Cricetinae</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytosol</style></keyword><keyword><style  face="normal" font="default" size="100%">genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Heart</style></keyword><keyword><style  face="normal" font="default" size="100%">Intracellular Fluid</style></keyword><keyword><style  face="normal" font="default" size="100%">isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Kidney</style></keyword><keyword><style  face="normal" font="default" size="100%">Kinetics</style></keyword><keyword><style  face="normal" font="default" size="100%">metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Methionine</style></keyword><keyword><style  face="normal" font="default" size="100%">Muscle,Smooth,Vascular</style></keyword><keyword><style  face="normal" font="default" size="100%">Ouabain</style></keyword><keyword><style  face="normal" font="default" size="100%">pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">potassium</style></keyword><keyword><style  face="normal" font="default" size="100%">sodium</style></keyword><keyword><style  face="normal" font="default" size="100%">Sodium-Calcium Exchanger</style></keyword><keyword><style  face="normal" font="default" size="100%">Transfection</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1993</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1993/04//</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">PM:8476012</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">264</style></volume><pages><style face="normal" font="default" size="100%">C1066 - C1074</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A line of Chinese hamster ovary (CHO) cells called CK1.4 was produced by transfection with the gene for the bovine cardiac Na(+)-Ca2+ exchanger. CK1.4 cells stably expressed substantial exchange activity and exchanger protein as shown by immunoprecipitation. Exchange activity was quantified as 45Ca2+ influx that depended on both increasing intracellular Na+ and lowering the concentration of external Na+. Replacing external Na+ with K+ slightly increased 45Ca2+ uptake by CK1.4 cells with basal Na+ and greatly increased 45Ca2+ uptake by Na(+)-loaded cells. Neither exchange activity nor exchanger protein was detected in the nontransfected parental line. By contrast to CK1.4 cells, replacing external Na+ with K+ decreased 45Ca2+ uptake in the nontransfected cells whether or not they were Na+ loaded. Changes in cytosolic free Ca2+ determined with fura-2 were consistent with the 45Ca2+ uptake data. Analysis of poly(A)(+)-RNA by Northern blot confirmed that CK1.4 cells, but not the parental line, expressed the exchanger. Expression of the exchanger was also observed in aortic myocytes and a renal epithelial cell line (LLC-MK2) but not in other lines of renal epithelial cells (MDCK, LLC-PK1) or human dermal fibroblasts. The cardiac exchanger produced substantial 45Ca2+ efflux from CK1.4 cells in response to hormone-evoked release of stored Ca2+. CK1.4 cells are an attractive model for studies of the regulation of the cardiac exchanger because they stably express sufficient exchanger for biochemical and immunological analysis</style></abstract><issue><style face="normal" font="default" size="100%">4 Pt 1</style></issue><notes><style face="normal" font="default" size="100%">DA - 19930517IS - 0002-9513 (Print)IS - 0002-9513 (Linking)LA - engPT - Journal ArticlePT - Research Support, U.S. Gov't, P.H.SRN - 0 (Calcium Radioisotopes)RN - 0 (Carrier Proteins)RN - 0 (Sodium-Calcium Exchanger)RN - 5ACL011P69 (Ouabain)RN - 9NEZ333N27 (Sodium)RN - AE28F7PNPL (Methionine)RN - RWP5GA015D (Potassium)RN - SY7Q814VUP (Calcium)SB - IM</style></notes></record></records></xml>