@article {16385,
	title = {Microcollimator for micrometer-wide stripe irradiation of cells using 20-30 keV X rays.},
	journal = {Radiat Res},
	volume = {172},
	year = {2009},
	month = {2009 Aug},
	pages = {252-9},
	abstract = {Abstract Pataky, K., Villanueva, G., Liani, A., Zgheib, O., Jenkins, N., Halazonetis, D. J., Halazonetis, T. D. and Brugger, J. Microcollimator for Micrometer-Wide Stripe Irradiation of Cells Using 20-30 keV X Rays. Radiat. Res. 172, 252-259 (2009). The exposure of subnuclear compartments of cells to ionizing radiation is currently not trivial. We describe here a collimator for micrometer-wide stripe irradiation designed to work with conventional high-voltage X-ray tubes and cells cultured on standard glass cover slips. The microcollimator was fabricated by high-precision silicon micromachining and consists of X-ray absorbing chips with grooves of highly controlled depths, between 0.5-10 microm, along their surfaces. These grooves form X-ray collimating slits when the chips are stacked against each other. The use of this device for radiation biology was examined by irradiating human cells with X rays having energies between 20-30 keV. After irradiation, p53 binding protein 1 (53BP1), a nuclear protein that is recruited at sites of DNA double-strand breaks, clustered in lines corresponding to the irradiated stripes.},
	keywords = {Cell Culture Techniques, Cell Line, Tumor, Cell Survival, Dose-Response Relationship, Radiation, Equipment Design, Equipment Failure Analysis, Humans, Miniaturization, Osteosarcoma, Radiation Dosage, Radiation Equipment and Supplies, X-Rays},
	issn = {0033-7587},
	doi = {10.1667/RR1483.1},
	author = {Pataky, Kristopher and Villanueva, Guillermo and Liani, Andre and Zgheib, Omar and Jenkins, Nathan and Halazonetis, Demetrios J and Halazonetis, Thanos D and Brugger, Juergen}
}
