<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pataky, Kristopher</style></author><author><style face="normal" font="default" size="100%">Villanueva, Guillermo</style></author><author><style face="normal" font="default" size="100%">Liani, Andre</style></author><author><style face="normal" font="default" size="100%">Zgheib, Omar</style></author><author><style face="normal" font="default" size="100%">Jenkins, Nathan</style></author><author><style face="normal" font="default" size="100%">Halazonetis, Demetrios J</style></author><author><style face="normal" font="default" size="100%">Halazonetis, Thanos D</style></author><author><style face="normal" font="default" size="100%">Brugger, Juergen</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Microcollimator for micrometer-wide stripe irradiation of cells using 20-30 keV X rays.</style></title><secondary-title><style face="normal" font="default" size="100%">Radiat Res</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Radiat. Res.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cell Culture Techniques</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Line, Tumor</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Survival</style></keyword><keyword><style  face="normal" font="default" size="100%">Dose-Response Relationship, Radiation</style></keyword><keyword><style  face="normal" font="default" size="100%">Equipment Design</style></keyword><keyword><style  face="normal" font="default" size="100%">Equipment Failure Analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Miniaturization</style></keyword><keyword><style  face="normal" font="default" size="100%">Osteosarcoma</style></keyword><keyword><style  face="normal" font="default" size="100%">Radiation Dosage</style></keyword><keyword><style  face="normal" font="default" size="100%">Radiation Equipment and Supplies</style></keyword><keyword><style  face="normal" font="default" size="100%">X-Rays</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2009 Aug</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">172</style></volume><pages><style face="normal" font="default" size="100%">252-9</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Abstract Pataky, K., Villanueva, G., Liani, A., Zgheib, O., Jenkins, N., Halazonetis, D. J., Halazonetis, T. D. and Brugger, J. Microcollimator for Micrometer-Wide Stripe Irradiation of Cells Using 20-30 keV X Rays. Radiat. Res. 172, 252-259 (2009). The exposure of subnuclear compartments of cells to ionizing radiation is currently not trivial. We describe here a collimator for micrometer-wide stripe irradiation designed to work with conventional high-voltage X-ray tubes and cells cultured on standard glass cover slips. The microcollimator was fabricated by high-precision silicon micromachining and consists of X-ray absorbing chips with grooves of highly controlled depths, between 0.5-10 microm, along their surfaces. These grooves form X-ray collimating slits when the chips are stacked against each other. The use of this device for radiation biology was examined by irradiating human cells with X rays having energies between 20-30 keV. After irradiation, p53 binding protein 1 (53BP1), a nuclear protein that is recruited at sites of DNA double-strand breaks, clustered in lines corresponding to the irradiated stripes.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/19630530?dopt=Abstract</style></custom1></record></records></xml>