@article {41220,
	title = {Distinct trafficking routes of polarized and non-polarized membrane cargoes in Aspergillus nidulans .},
	journal = {Elife},
	volume = {13},
	year = {2024},
	month = {2024 Oct 21},
	abstract = {Membrane proteins are sorted to the plasma membrane (PM) via Golgi-dependent trafficking. However, our recent studies challenged the essentiality of Golgi in the biogenesis of specific transporters. Here, we investigate the trafficking mechanisms of membrane proteins by following the localization of the polarized R-SNARE SynA versus the non-polarized transporter UapA, synchronously co-expressed in wild-type or isogenic genetic backgrounds repressible for conventional cargo secretion. In wild-type, the two cargoes dynamically label distinct secretory compartments, highlighted by the finding that, unlike SynA, UapA does not colocalize with the late-Golgi. In line with early partitioning into distinct secretory carriers, the two cargoes collapse in distinct ERES in a background. Trafficking via distinct cargo-specific carriers is further supported by showing that repression of proteins essential for conventional cargo secretion does not affect UapA trafficking, while blocking SynA secretion. Overall, this work establishes the existence of distinct, cargo-dependent, trafficking mechanisms, initiating at ERES and being differently dependent on Golgi and SNARE interactions.},
	issn = {2050-084X},
	doi = {10.7554/eLife.103355},
	author = {Sagia, Georgia Maria and Georgiou, Xenia and Chamilos, Georgios and Diallinas, George and Dimou, Sofia}
}
