@article {7410,
	title = {The AP-2 complex has a specialized clathrin-independent role in apical endocytosis and polar growth in fungi.},
	journal = {Elife},
	volume = {6},
	year = {2017},
	month = {2017 Feb 21},
	abstract = {Filamentous fungi provide excellent systems for investigating the role of the AP-2 complex in polar growth. Using Aspergillus nidulans, we show that AP-2 has a clathrin-independent essential role in polarity maintenance and growth. This is in line with a sequence analysis showing that the AP-2 β subunit (β2) of higher fungi lacks a clathrin-binding domain, and experiments showing that AP-2 does not co-localize with clathrin. We provide genetic and cellular evidence that AP-2 interacts with endocytic markers SlaB(End4) and SagA(End3) and the lipid flippases DnfA and DnfB in the sub-apical collar region of hyphae. The role of AP-2 in the maintenance of proper apical membrane lipid and cell wall composition is further supported by its functional interaction with BasA (sphingolipid biosynthesis) and StoA (apical sterol-rich membrane domains), and its essentiality in polar deposition of chitin. Our findings support that the AP-2 complex of dikarya has acquired, in the course of evolution, a specialized clathrin-independent function necessary for fungal polar growth.},
	issn = {2050-084X},
	doi = {10.7554/eLife.20083},
	author = {Martzoukou, Olga and Amillis, Sotiris and Zervakou, Amalia and Christoforidis, Savvas and Diallinas, George}
}
