@article {20356,
	title = {HIV-1 transmission networks across Cyprus (2010-2012)},
	journal = {PLoS OnePLoS OnePLoS One},
	volume = {13},
	number = {4},
	year = {2018},
	note = {1932-6203Kostrikis, Leondios GOrcid: 0000-0002-5340-7109Hezka, JohanaStylianou, Dora CKostaki, EvangeliaAndreou, MariaKousiappa, IoannaParaskevis, DimitriosDemetriades, IoannisJournal ArticlePLoS One. 2018 Apr 23;13(4):e0195660. doi: 10.1371/journal.pone.0195660. eCollection 2018.},
	pages = {e0195660},
	edition = {2018/04/24},
	abstract = {A molecular epidemiology study of HIV-1 infection was conducted in one hundred diagnosed and untreated HIV-1-infected patients in Cyprus between 2010 and 2012, representing 65.4\% of all the reported HIV-1 infections in Cyprus in this three-year period, using a previously defined enrolment strategy. Eighty-two patients were newly diagnosed (genotypic drug resistance testing within six months from diagnosis), and eighteen patients were HIV-1 diagnosed for a longer period or the diagnosis date was unknown. Phylogenetic trees of the pol sequences obtained in this study with reference sequences indicated that subtypes B and A1 were the most common subtypes present and accounted for 41.0 and 19.0\% respectively, followed by subtype C (7.0\%), F1 (8.0\%), CRF02_AG (4.0\%), A2 (2.0\%), other circulating recombinant forms (CRFs) (7.0\%) and unknown recombinant forms (URFs) (12\%). Most of the newly-diagnosed study subjects were Cypriots (63\%), males (78\%) with median age 39 (Interquartile Range, IQR 33-48) reporting having sex with other men (MSM) (51\%). A high rate of clustered transmission of subtype B drug-sensitive strains to reverse transcriptase and protease inhibitors was observed among MSM, twenty-eight out of forty-one MSM study subjects (68.0\%) infected were implicated in five transmission clusters, two of which are sub-subtype A1 and three of which are subtype B strains. The two largest MSM subtype B clusters included nine and eight Cypriot men, respectively, living in all major cities in Cyprus. There were only three newly diagnosed patients with transmitted drug resistant HIV-1 strains, one study subject from the United Kingdom infected with subtype B strain and one from Romania with sub-subtype A2 strain, both with PI drug resistance mutation M46L and one from Greece with sub-subtype A1 with non-nucleoside reverse transcriptase inhibitors (NNRTI) drug resistance mutation K103N.},
	keywords = {*HIV-1/genetics, Adolescent, Adult, Aged, Cyprus/epidemiology, Drug Resistance, Viral/genetics, Female, Geography, Medical, HIV Infections/*transmission, Homosexuality, Male, Humans, Male, Middle Aged, molecular epidemiology, Phylogeny, Prospective Studies, Young Adult},
	isbn = {1932-6203},
	author = {Kostrikis, L G and Hezka, J and Stylianou, D. C. and E Kostaki and Andreou, M. and Kousiappa, I and Paraskevis, D and Demetriades, I}
}
