<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Drosatos, K.</style></author><author><style face="normal" font="default" size="100%">Sanoudou, D.</style></author><author><style face="normal" font="default" size="100%">Kypreos, K. E.</style></author><author><style face="normal" font="default" size="100%">Kardassis, D.</style></author><author><style face="normal" font="default" size="100%">Zannis, V. I.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A dominant negative form of the transcription factor c-Jun affects genes that have opposing effects on lipid homeostasis in mice</style></title><secondary-title><style face="normal" font="default" size="100%">J Biol ChemJ Biol ChemJ Biol Chem</style></secondary-title><alt-title><style face="normal" font="default" size="100%">The Journal of biological chemistry</style></alt-title><short-title><style face="normal" font="default" size="100%">The Journal of biological chemistryThe Journal of biological chemistry</style></short-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*Genes, Dominant</style></keyword><keyword><style  face="normal" font="default" size="100%">*Homeostasis/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">*Lipid Metabolism/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">*Signal Transduction/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Adenoviridae</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Apolipoproteins E/biosynthesis/deficiency</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Line, Tumor</style></keyword><keyword><style  face="normal" font="default" size="100%">Cholesterol/blood</style></keyword><keyword><style  face="normal" font="default" size="100%">Dyslipidemias/blood/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Genome</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">JNK Mitogen-Activated Protein Kinases/genetics/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Liver/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice, Knockout</style></keyword><keyword><style  face="normal" font="default" size="100%">Proto-Oncogene Proteins c-jun/*biosynthesis/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">RNA, Messenger/biosynthesis/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Stearoyl-CoA Desaturase/genetics/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Triglycerides/blood</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jul 6</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">27</style></number><volume><style face="normal" font="default" size="100%">282</style></volume><pages><style face="normal" font="default" size="100%">19556-64</style></pages><isbn><style face="normal" font="default" size="100%">0021-9258 (Print)0021-9258 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">c-Jun is a transcription factor activated by phosphorylation by the stress-activated protein kinase/c-Jun N-terminal kinase pathway in response to extracellular signals and cytokines. We show that adenovirus-mediated gene transfer of the dominant negative form of c-Jun (dn-c-Jun) in C57BL/6 mice increased greatly apoE hepatic mRNA and plasma levels, increased plasma cholesterol, triglyceride, and very low density lipoprotein levels, and resulted in the accumulation of discoidal high density lipoprotein particles. A similar but more severe phenotype was generated by overexpression of the mouse apoE in C57BL/6 mice, suggesting that dyslipidemia induced by dn-c-Jun was the result of apoE overexpression. Unexpectedly, infection of apoE(-/-) mice with adenovirus expressing dn-c-Jun reduced plasma cholesterol by 70%, suggesting that dn-c-Jun affected other genes that control plasma cholesterol levels. To identify these genes, we performed whole genome expression analysis (34,000 genes) of isolated livers from two groups of five apoE(-/-) mice, infected with adenoviruses expressing either the dn-c-Jun or the green fluorescence protein. Bioinformatic analysis and Northern blotting validation revealed that dn-c-Jun increased 40-fold the apoE mRNA and reduced by 70% the Scd-1 (stearoyl-CoA-desaturase 1) mRNA. The involvement of Scd-1 in lowering plasma cholesterol was confirmed by restoration of high cholesterol levels of apoE(-/-) mice following coinfection with adenoviruses expressing dn-c-Jun and Scd-1. In conclusion, dn-c-Jun appears to trigger two opposing events in mice that affect plasma cholesterol and triglyceride levels as follows: one results in apoE overexpression and triggers dyslipidemia and the other results in inhibition of Scd-1 and offsets dyslipidemia.</style></abstract><accession-num><style face="normal" font="default" size="100%">17456467</style></accession-num><notes><style face="normal" font="default" size="100%">Drosatos, KonstantinosSanoudou, DespinaKypreos, Kyriakos EKardassis, DimitrisZannis, Vassilis IengR01 HL048739-11/HL/NHLBI NIH HHS/HL48739/HL/NHLBI NIH HHS/R01 HL048739/HL/NHLBI NIH HHS/R01 HL033952/HL/NHLBI NIH HHS/R01 HL068216/HL/NHLBI NIH HHS/R01 HL068216-05A1/HL/NHLBI NIH HHS/HL33952/HL/NHLBI NIH HHS/Research Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't2007/04/26 09:00J Biol Chem. 2007 Jul 6;282(27):19556-64. doi: 10.1074/jbc.M700986200. Epub 2007 Apr 24.</style></notes><custom2><style face="normal" font="default" size="100%">2745720</style></custom2><auth-address><style face="normal" font="default" size="100%">Department of Basic Sciences, University of Crete Medical School, Heraklion GR-71110, Greece.</style></auth-address></record></records></xml>