<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tomczak, K. K.</style></author><author><style face="normal" font="default" size="100%">Marinescu, V. D.</style></author><author><style face="normal" font="default" size="100%">Ramoni, M. F.</style></author><author><style face="normal" font="default" size="100%">Sanoudou, D.</style></author><author><style face="normal" font="default" size="100%">Montanaro, F.</style></author><author><style face="normal" font="default" size="100%">Han, M.</style></author><author><style face="normal" font="default" size="100%">Kunkel, L. M.</style></author><author><style face="normal" font="default" size="100%">Kohane, I. S.</style></author><author><style face="normal" font="default" size="100%">Beggs, A. H.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Expression profiling and identification of novel genes involved in myogenic differentiation</style></title><secondary-title><style face="normal" font="default" size="100%">FASEB JFASEB JFASEB J</style></secondary-title><alt-title><style face="normal" font="default" size="100%">FASEB journal : official publication of the Federation of American Societies for Experimental Biology</style></alt-title><short-title><style face="normal" font="default" size="100%">FASEB journal : official publication of the Federation of American Societies for Experimental BiologyFASEB journal : official publication of the Federation of American Societies for Experimental Biology</style></short-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*Gene Expression Profiling</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Cycle/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Differentiation/*genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Division/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Line</style></keyword><keyword><style  face="normal" font="default" size="100%">Cluster analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Down-Regulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Expressed Sequence Tags</style></keyword><keyword><style  face="normal" font="default" size="100%">Genes/*genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice</style></keyword><keyword><style  face="normal" font="default" size="100%">Muscle Development</style></keyword><keyword><style  face="normal" font="default" size="100%">Muscles/cytology/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Myoblasts/*cytology/*metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">RNA, Messenger/genetics/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Transcription, Genetic</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Feb</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">2</style></number><volume><style face="normal" font="default" size="100%">18</style></volume><pages><style face="normal" font="default" size="100%">403-5</style></pages><isbn><style face="normal" font="default" size="100%">1530-6860 (Electronic)0892-6638 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Skeletal muscle differentiation is a complex, highly coordinated process that relies on precise temporal gene expression patterns. To better understand this cascade of transcriptional events, we used expression profiling to analyze gene expression in a 12-day time course of differentiating C2C12 myoblasts. Cluster analysis specific for time-ordered microarray experiments classified 2895 genes and ESTs with variable expression levels between proliferating and differentiating cells into 22 clusters with distinct expression patterns during myogenesis. Expression patterns for several known and novel genes were independently confirmed by real-time quantitative RT-PCR and/or Western blotting and immunofluorescence. MyoD and MEF family members exhibited unique expression kinetics that were highly coordinated with cell-cycle withdrawal regulators. Among genes with peak expression levels during cell cycle withdrawal were Vcam1, Itgb3, Itga5, Vcl, as well as Ptger4, a gene not previously associated with the process of myogenesis. One interesting uncharacterized transcript that is highly induced during myogenesis encodes several immunoglobulin repeats with sequence similarity to titin, a large sarcomeric protein. These data sets identify many additional uncharacterized transcripts that may play important functions in muscle cell proliferation and differentiation and provide a baseline for comparison with C2C12 cells expressing various mutant genes involved in myopathic disorders.</style></abstract><accession-num><style face="normal" font="default" size="100%">14688207</style></accession-num><notes><style face="normal" font="default" size="100%">Tomczak, Kinga KMarinescu, Voichita DRamoni, Marco FSanoudou, DespinaMontanaro, FedericaHan, MeiKunkel, Louis MKohane, Isaac SBeggs, Alan HengP01 NS040828/NS/NINDS NIH HHS/2003/12/23 05:00FASEB J. 2004 Feb;18(2):403-5. doi: 10.1096/fj.03-0568fje. Epub 2003 Dec 19.</style></notes><auth-address><style face="normal" font="default" size="100%">Genetics Division, Children's Hospital, Boston, Massachusetts 02115, USA.</style></auth-address></record></records></xml>