@article {11587,
	title = {Age-dependent changes in 8-oxoguanine-DNA glycosylase activity are modulated by adaptive responses to physical exercise in human skeletal muscle.},
	journal = {Free Radic Biol Med},
	volume = {51},
	year = {2011},
	month = {2011 Jul 15},
	pages = {417-23},
	abstract = {8-Oxo-7,8-dihydroguanine (8-oxoG) accumulates in the genome over time and is believed to contribute to the development of aging characteristics of skeletal muscle and various aging-related diseases. Here, we show a significantly increased level of intrahelical 8-oxoG and 8-oxoguanine-DNA glycosylase (OGG1) expression in aged human skeletal muscle compared to that of young individuals. In response to exercise, the 8-oxoG level was lastingly elevated in sedentary young and old subjects, but returned rapidly to preexercise levels in the DNA of physically active individuals independent of age. 8-OxoG levels in DNA were inversely correlated with the abundance of acetylated OGG1 (Ac-OGG1), but not with total OGG1, apurinic/apyrimidinic endonuclease 1 (APE1), or Ac-APE1. The actual Ac-OGG1 level was linked to exercise-induced oxidative stress, as shown by changes in lipid peroxide levels and expression of Cu,Zn-SOD, Mn-SOD, and SIRT3, as well as the balance between acetyltransferase p300/CBP and deacetylase SIRT1, but not SIRT6 expression. Together these data suggest that that acetylated form of OGG1, and not OGG1 itself, correlates inversely with the 8-oxoG level in the DNA of human skeletal muscle, and the Ac-OGG1 level is dependent on adaptive cellular responses to physical activity, but is age independent.},
	keywords = {Adult, Age Factors, Aged, Base Sequence, DNA Glycosylases, DNA Primers, Exercise, Guanine, Humans, Middle Aged, Muscle, Skeletal, Reverse Transcriptase Polymerase Chain Reaction},
	issn = {1873-4596},
	doi = {10.1016/j.freeradbiomed.2011.04.018},
	author = {Radak, Zsolt and Bori, Zoltan and Koltai, Erika and Fatouros, Ioannis G and Jamurtas, Athanasios Z and Douroudos, Ioannis I and Terzis, Gerasimos and Nikolaidis, Michalis G and Chatzinikolaou, Athanasios and Sovatzidis, Apostolos and Kumagai, Shuzo and Naito, Hisahi and Boldogh, Istvan}
}
