<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vontzalidis, Adamantios</style></author><author><style face="normal" font="default" size="100%">Terzis, Gerasimos</style></author><author><style face="normal" font="default" size="100%">Manta, Panagiota</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Increased dysferlin expression in Duchenne muscular dystrophy.</style></title><secondary-title><style face="normal" font="default" size="100%">Anal Quant Cytopathol Histpathol</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Anal Quant Cytopathol Histpathol</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Biopsy</style></keyword><keyword><style  face="normal" font="default" size="100%">Child</style></keyword><keyword><style  face="normal" font="default" size="100%">Child, Preschool</style></keyword><keyword><style  face="normal" font="default" size="100%">Dysferlin</style></keyword><keyword><style  face="normal" font="default" size="100%">Dystrophin</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunoblotting</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunohistochemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Male</style></keyword><keyword><style  face="normal" font="default" size="100%">Membrane Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Muscle Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Muscle, Skeletal</style></keyword><keyword><style  face="normal" font="default" size="100%">Muscular Dystrophy, Duchenne</style></keyword><keyword><style  face="normal" font="default" size="100%">Sarcolemma</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2014 Feb</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">36</style></volume><pages><style face="normal" font="default" size="100%">15-22</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">OBJECTIVE: To investigate dysferlin expression in muscle biopsies from patients with Duchenne muscular dystrophy (DMD). Dysferlin is known to have a role in the process of membrane fusion and muscle membrane repair in skeletal muscle fibers.

STUDY DESIGN: We analyzed 20 muscle biopsy samples of DMD patients with immunohistochemical techniques to determine the expression of dysferlin. Immunoblotting was performed to assess dysferlin abundance in dystrophic muscle.

RESULTS: Dysferlin showed various immunostaining patterns in dystrophic muscle, including reduced, normal, or enhanced sarcolemmal expression and intracellular immunostaining of the protein. Immunoblotting revealed that dysferlin was upregulated in 15 out of the 20 samples (75%). The abundance of the protein was analogous to the number of fibers with enhanced sarcolemmal expression of the protein.

CONCLUSION: These data suggest that although dysferlin is not an integral part of the dystrophin-glycoprotein complex, its expression is altered in Duchenne muscular dystrophy.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/24902367?dopt=Abstract</style></custom1></record></records></xml>