<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Natalia Kamperi</style></author><author><style face="normal" font="default" size="100%">Geballa-Koukoula, Ariadni</style></author><author><style face="normal" font="default" size="100%">Sophia Komninou</style></author><author><style face="normal" font="default" size="100%">Iraklis Salvanos</style></author><author><style face="normal" font="default" size="100%">Ioanna Ioannou</style></author><author><style face="normal" font="default" size="100%">Foteini Theiakou</style></author><author><style face="normal" font="default" size="100%">Panderi, Irene</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Development and validation of a HILIC–ESI/MS method for quantitation of three oxicams and 5-hydroxypiroxicam in human breast milk</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Chromatography B</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Breast milk</style></keyword><keyword><style  face="normal" font="default" size="100%">HILIC</style></keyword><keyword><style  face="normal" font="default" size="100%">LC-MS</style></keyword><keyword><style  face="normal" font="default" size="100%">NSAIDs</style></keyword><keyword><style  face="normal" font="default" size="100%">Oxicams</style></keyword><keyword><style  face="normal" font="default" size="100%">Protein precipitation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2026</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.sciencedirect.com/science/article/pii/S1570023226000620</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">1274</style></volume><pages><style face="normal" font="default" size="100%">124973</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Human breast milk is a complex biological matrix that plays a central role in infant nutrition and development. It may also serve as a route of exposure to pharmaceutical residues following maternal drug use. Quantitative data on the transfer of non-steroidal anti-inflammatory drugs (NSAIDs) into breast milk are essential to support their safe use during lactation, however, available analytical methods remain limited. This study reports the development and validation of a rapid and sensitive hydrophilic interaction liquid chromatography–electrospray ionization mass spectrometry (HILIC–ESI/MS) method for the simultaneous quantitation of tenoxicam, meloxicam, piroxicam, and the metabolite 5′-hydroxypiroxicam in human breast milk. Sample preparation involves simple protein precipitation procedure using only 25 μL of human breast milk. Chromatographic separation is achieved within 8 min. The method was validated over the range of 20 to 2000 ng mL−1, with a limit of quantitation of 20 ng mL−1 and correlation coefficients greater than 0.998. This is the first HILIC–ESI/MS method for the simultaneous quantitation of the targeted analytes in human breast milk. The validated HILIC–ESI/MS method was subsequently applied to screen breast milk samples collected from ten lactating women donating to a hospital milk bank. Although oxicam NSAIDs were not detected in this cohort, the method is well-suited for targeted screening of human milk in clinical or surveillance settings, including milk banks and pharmacovigilance studies.</style></abstract></record></records></xml>