@article {5154,
	title = {Comparative evaluation of conventional and real-time PCR assays for detecting Bacteroides fragilis in clinical samples.},
	journal = {J Clin Microbiol},
	volume = {51},
	year = {2013},
	month = {2013 May},
	pages = {1593-5},
	abstract = {A conventional PCR and a real-time PCR for detecting Bacteroides fragilis were evaluated against clinical specimens. Analytical sensitivities were 100 and 40 fg of DNA, respectively. Detection limits were 100 and 10 CFU/ml, respectively. A total of six culture-negative specimens were positive by PCR. Altering the gold standard from "positive culture" to "positive culture or both PCR assays positive" resulted in sensitivities of 91.7\% and 100\%, respectively, and in specificities of 100\% and 98.6\%, respectively.},
	keywords = {Bacteroides fragilis, Bacteroides Infections, DNA, Bacterial, Feces, Humans, Limit of Detection, Real-Time Polymerase Chain Reaction, RNA, Ribosomal, 16S, Sensitivity and Specificity},
	issn = {1098-660X},
	doi = {10.1128/JCM.00449-13},
	author = {Papaparaskevas, Joseph and Mela, Victoria and Houhoula, Dimitra P and Pantazatou, Angeliki and Petrikkos, Georgios L and Tsakris, Athanassios}
}
