@article {Georgousaki2020,
	title = {{Screening for tyrosinase inhibitors from actinomycetes; identification of trichostatin derivatives from Streptomyces sp. CA-129531 and scale up production in bioreactor}},
	journal = {Bioorganic {\&} Medicinal Chemistry Letters},
	number = {January},
	year = {2020},
	month = {jan},
	pages = {126952},
	publisher = {Elsevier},
	abstract = {In the course of a primary screening of 614 microbial actinomycete extracts for the discovery of tyrosinase inhibitors, the EtOAc extract of the fermentation broth of the strain Streptomyces sp. CA-129531 isolated from a Martinique sample, exhibited in cell free and cell-based assays the most promising activity (IC50 value of 63 $μ$g/mL). Scaled-up production in a bioreactor led to the isolation of one new trichostatic acid analogue, namely trichostatic acid B (1), along with six known trichostatin derivatives (2-7), four diketopiperazines (8-11), two butyrolactones (12-13) and one hydroxamic acid siderophore (14). Among them, trichostatin A (4) showed six times stronger anti-tyrosinase activity (IC50 2.18 $μ$Μ) than kojic acid (IC50 14.07 $μ$Μ) used as a positive control. Deoxytrichostatin A (6) displayed also strong inhibitory activity against tyrosinase (IC50 19.18 $μ$Μ). Trichostatin A production in bioreactor started together with the exponential phase of growth (day 4) and the maximum concentration was reached at day 9 (2.67 {\textpm} 0.13 $μ$g/mL). Despite the cytotoxicity of some individual components, no cytotoxic effect on HepG2, A2058, A549, MCF-7 and MIA PaCa-2 cell lines was found for the EtOAc extract (IC50 {\textgreater} 2.84 mg/mL), while it found no cytotoxic against BG fibroblasts at the concentrations were it exerted whitening effect, reassuring its safety and great tyrosinase inhibitory potential.},
	keywords = {Bioreactor, Enzyme kinetics studies, Secondary metabolites, streptomyces sp, Streptomyces sp., Trichostatin derivatives, Tyrosinase inhibitors},
	issn = {0960894X},
	doi = {10.1016/j.bmcl.2020.126952},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/?term=10.1016{\%}2Fj.bmcl.2020.126952. https://linkinghub.elsevier.com/retrieve/pii/S0960894X20300056},
	author = {Georgousaki, Katerina and Tsafantakis, Nikolaos and Gumeni, Sentiljana and Gonzalez, Ignacio and Mackenzie, Thomas Andrew and Reyes, Fernando and Lambert, Carole and Trougakos, Ioannis P. and Genilloud, Olga and Fokialakis, Nikolas}
}
