@article {Samy2019,
	title = {{Osmanicin, a Polyketide Alkaloid Isolated from Streptomyces osmaniensis CA-244599 Inhibits Elastase in Human Fibroblasts}},
	journal = {Molecules},
	volume = {24},
	number = {12},
	year = {2019},
	month = {jun},
	pages = {2239},
	abstract = {The strain Streptomyces osmaniensis CA-244599 isolated from the Comoros islands was submitted to liquid-state fermentation coupled to in situ solid-phase extraction with amberlite XAD-16 resin. Elution of the trapped compounds on the resin beads by ethyl acetate afforded seven metabolites, osmanicin (1), streptazolin (2), streptazone C (3), streptazone B1 (4), streptenol C (5), nocardamine (6) and desmethylenylnocardamine (7). Osmanicin (1) is a newly reported unusual scaffold combining streptazolin (2) and streptazone C (3) through a Diels-Alder type reaction. Experimental evidence excluded the spontaneous formation of 1 from 2 and 3. The isolated compounds were evaluated for their ability to inhibit elastase using normal human diploid fibroblasts. Compound 1 exhibited the most potent activity with an IC50 of 3.7 $μ$M.},
	keywords = {alkaloids, elastase, in situ solid-phase extraction, osmanicin, streptomyces osmaniensis},
	issn = {1420-3049},
	doi = {10.3390/molecules24122239},
	url = {https://www.mdpi.com/1420-3049/24/12/2239},
	author = {Samy, Mamdouh N. and {Le Goff}, G{\'e}raldine and Lopes, Philippe and Georgousaki, Katerina and Gumeni, Sentiljana and Almeida, Celso and Gonz{\'a}lez, Ignacio and Genilloud, Olga and Trougakos, Ioannis and Fokialakis, Nikolas and Ouazzani, Jamal}
}
