@article {Kastritis2020,
	title = {{Next generation flow cytometry for MRD detection in patients with AL amyloidosis}},
	journal = {Amyloid},
	year = {2020},
	month = {aug},
	pages = {1{\textendash}5},
	publisher = {Taylor {\&} Francis},
	abstract = {The treatment of AL amyloidosis aims to eradicate the plasma cell clone and eliminate toxic free light chain production. Only in a minority of patients the plasma cell clone is completely eradicated; residual light chain production may still exist while clonal relapse may occur. We used sensitive next-generation flow cytometry (NGF) to detect minimal residual disease (MRD) in AL amyloidosis patients at complete haematologic response. MRD evaluation was feasible in 51 of 52 (98{\%}) tested patients and at a median sensitivity of 2.3 {\texttimes} 10-6 MRD was undetectable in 23 (45{\%}). An organ response occurred in 86{\%} of MRDneg vs 77{\%} in MRDpos; renal response in 15/17(88{\%}) of MRDneg vs in 14/16(87.5{\%}) of MRDpos and cardiac response in 10/10(100{\%}) of MRDneg vs 11/15(73{\%}) of MRDpos patients. After a median follow-up of 24 months post MRD testing, no MRDneg patient had a haematologic relapse vs 6/28(21{\%}) MRDpos (p =.029). Pooling haematologic and organ progressions, 9 (32{\%}) MRDpos patients had disease progression vs only 1 (4{\%}) MRDneg patient (p =.026). In conclusion, MRD detection using NGF has profound clinical implications, so that AL patients with undetectable MRD have a very high probability of organ response and a very low probability of haematologic relapse.},
	keywords = {EuroFlow, free light chains, minimal residual disease, next generation flow cytometry, NTproBNP},
	issn = {1350-6129},
	doi = {10.1080/13506129.2020.1802713},
	url = {https://doi.org/10.1080/13506129.2020.1802713 https://www.tandfonline.com/doi/full/10.1080/13506129.2020.1802713},
	author = {Kastritis, Esftathios and Kostopoulos, Ioannis V. and Theodorakakou, Foteini and Fotiou, Despina and Gavriatopoulou, Maria and Migkou, Magdalini and Tselegkidi, Maria Irini and Roussou, Maria and Papathoma, Alexandra and Eleutherakis-Papaioakovou, Evangelos and Dialoupi, Ioanna and Kanellias, Nikolaos and Ntalianis, Argyrios and Rousakis, Pantelis and Trougakos, Ioannis P. and Tsitsilonis, Ourania and Gakiopoulou, Charikleia and Terpos, Evangelos and Dimopoulos, Meletios A}
}
