<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Georgousaki, Katerina</style></author><author><style face="normal" font="default" size="100%">Tsafantakis, Nikolaos</style></author><author><style face="normal" font="default" size="100%">Gumeni, Sentiljana</style></author><author><style face="normal" font="default" size="100%">González-Menéndez, Victor</style></author><author><style face="normal" font="default" size="100%">de Pedro, Nuria</style></author><author><style face="normal" font="default" size="100%">Tormo, José Rubén</style></author><author><style face="normal" font="default" size="100%">Almeida, Celso</style></author><author><style face="normal" font="default" size="100%">Lambert, Carole</style></author><author><style face="normal" font="default" size="100%">Genilloud, Olga</style></author><author><style face="normal" font="default" size="100%">Trougakos, Ioannis P.</style></author><author><style face="normal" font="default" size="100%">Fokialakis, Nikolas</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">{Cercospora sp. as a source of anti-aging polyketides targeting 26S proteasome and scale-up production in submerged bioreactor}</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Biotechnology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antioxidant activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Bioreactor</style></keyword><keyword><style  face="normal" font="default" size="100%">Cercospora</style></keyword><keyword><style  face="normal" font="default" size="100%">Filamentous fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">Polyketides</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteasome</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">aug</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://linkinghub.elsevier.com/retrieve/pii/S0168165619301798</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">January</style></number><publisher><style face="normal" font="default" size="100%">Elsevier</style></publisher><volume><style face="normal" font="default" size="100%">301</style></volume><pages><style face="normal" font="default" size="100%">88–96</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">From a large screening of microbial extracts for the discovery of proteasome modulating natural products, the fungal strain Cercospora sp. (CF-223709) was selected as the most promising for further investigation. Different liquid cultures of the strain were initially screened for their anti-oxidant activity (DPPH, ABTS) and for their cytotoxicity against the A2058, HepG2 and CCD25sk cell lines. A detailed chemical analysis and evaluation of the capacity to activate 26S-proteasome was followed for the most active extract. Three main polyketides were isolated and characterized by extensive analysis of NMR and HRMS spectra data as penialidine F (1), fulvic acid (2), and SB238569 (3). Fulvic acid showed the most significant anti-oxidant activity. Its IC50 value (8.16 $μ$M) against the ABTS radical resulted 3-fold lower than the standard trolox. Fulvic acid also demonstrated a significant effect on proteasome by enhancing the chymotrypsin- and caspase-like activities of the 26S proteasome of human fibroblasts by 71.43{%} and 37.5{%} at 1 $μ$M, respectively. Furthermore by scaling up the culture in a 30 L submerged bioreactor, Cercospora sp. produced up to 162.6 ± 1.3 mg of fulvic acid/L. Our findings suggest that CF-223709 can be a promising source of proteasome activating natural compounds.</style></abstract></record></records></xml>