@article { WOS:A1994PA98500014,
	title = {GENERAL TREATMENT OF COMPETITIVE-BINDING AS APPLIED TO THE POTENTIOMETRIC ION PROBE TECHNIQUE - APPLICATION TO THE INTERACTION OF NONSTEROIDAL ANTIINFLAMMATORY DRUGS WITH BOVINE SERUM-ALBUMIN},
	journal = {JOURNAL OF PHARMACEUTICAL SCIENCES},
	volume = {83},
	number = {8},
	year = {1994},
	month = {AUG},
	pages = {1150-1154},
	publisher = {AMER PHARMACEUTICAL ASSN},
	type = {Article},
	address = {2215 CONSTITUTION AVE NW, WASHINGTON, DC 20037},
	abstract = {The binding of naproxen, ketoprofen, phenylbutazone, salicyclic acid, azapropazone, and indobufen to bovine serum albumin was studied by applying the potentiometric ion probe technique. An ion-selective electrode for the ion probe 1-anilino-8-naphthalenesulfonate was utilized for the purposes of this study. A modified site-oriented competitive binding model was used for the estimation of the drugs{\textquoteright} binding parameters, considering different number of binding sites on the competing binding class(es) for the probe and the drug. Calculations v,ere based exclusively on the concentration data of the free probe. The model{\textquoteright}s ability for accurate estimations of binding parameters was evaluated by simulation studies. The following values of binding parameters were found at 25 degrees C for the drugs under study; naproxen, n(1) = 9.1, k(1) = 9.4 X 10(5) M(-1); ketoprofen, n(1) = 8.8, k(1) = 10.8 X 10(5) M(-1); phenylbutazone, n(1) = 3.2, k(1) = 1.4 X 10(5) M(-1); salicylic acid, n(1) = 2.6, k(1) = 1.8 X 10(5) M(-1), n(2) = 21.5, k(2) = 1.0 X 10(4) M(-1); azapropazone, n(1) = 0.5, k(1) = 7.8 X 10(5) M(-1), n(2) = 26.3, k(2) = 1.9 X 10(4) M(-1); indobufen, n(1) = 5.8, k(1) = 5.8 X 10(5) M(-1), n(2) = 19.9, k(2) = 3.8 X 10(5) M(-1), where n(i) the number of binding sites of the i class and k(i) the corresponding association constant.},
	issn = {0022-3549},
	doi = {10.1002/jps.2600830816},
	author = {ANGELAKOU, AT and Sideris, EE and Valsami, GN and Koupparis, MA and Macheras, PE}
}
