@article { WOS:A1994NF41200011,
	title = {AUTOMATED FLOW-INJECTION SERIAL DYNAMIC DIALYSIS TECHNIQUE IN THE STUDY OF DRUG-BINDING WITH CYCLODEXTRINS},
	journal = {ANALYTICA CHIMICA ACTA},
	volume = {289},
	number = {1},
	year = {1994},
	month = {APR 20},
	pages = {87-95},
	publisher = {ELSEVIER SCIENCE BV},
	type = {Article},
	address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS},
	abstract = {A flow-injection dynamic dialysis technique is presented for the determination of binding parameters of drugs to cyclodextrins (CDs). The automated system consists of a flow-injection unit, the sample loop of which is the receiving compartment of a dialyser unit, and a home made timing module for operation control through two flow switching solenoid valves. The procedure of binding studies is rapid and yields reproducible results. Binding parameters of CD-micromolecule complexes was calculated using the Scatchard model. Typical examples of the binding of p-nitrophenol with alpha-CD(K-as=1.56x10(3) M(-1) at pH 7.4 and 2.06x10(3) M(-1) at pH 9.0), salicylic acid with beta-CD (K-as=3.8 x 10(2) M(-1) at pH 1.5 and 51 M(-1) at pH 7.4) and ibuprofen with beta-CD (K-as=2.2x10(2) M(-1) at pH 2.5) are presented and the binding constants obtained are compared to literature values. 1:1 stoichiometry was found in all cases and within run precision ranged from 2 to 14\% R.S.D. The between run precision for the binding of p-nitrophenol to alpha-CD was 2\% (n=3).},
	keywords = {AUTOMATED SERIAL DYNAMIC DIALYSIS TECHNIQUE, cyclodextrins, DRUG BINDING TO CYCLODEXTRINS, FLOW INJECTION, Ibuprofen, P-NITROPHENOL, SALICYLIC ACID, Scatchard model},
	issn = {0003-2670},
	doi = {10.1016/0003-2670(94)80011-1},
	author = {Sideris, EE and Georgiou, C.A. and Koupparis, MA and Macheras, PE}
}
