@article { WOS:A1992KB94800008,
	title = {DETERMINATION OF ASSOCIATION CONSTANTS IN CYCLODEXTRIN DRUG COMPLEXATION USING THE SCATCHARD PLOT - APPLICATION TO BETA-CYCLODEXTRIN-ANILINONAPHTHALENESULFONATES},
	journal = {PHARMACEUTICAL RESEARCH},
	volume = {9},
	number = {12},
	year = {1992},
	month = {DEC},
	pages = {1568-1574},
	publisher = {PLENUM PUBL CORP},
	type = {Article},
	address = {233 SPRING ST, NEW YORK, NY 10013},
	abstract = {The appropriate Scatchard equation was developed for a system involving the formation of 1:1 and 1:2 substrate:cyclodextrin complexes. Simulation of this system was performed under the most common experimental conditions encountered in this type of study. The use of the equation allows for nonlinear least-squares estimation of the association constants. The interaction of the model compounds 1-anilino-8-naphthalenesulfonate (1,8-ANS) and 2(p-toluidinyl)-6-naphthalenesulfonate (2,6-TNS) with beta-cyclodextrin (beta-CD) was used to evaluate the theoretical model. Binding experiments were performed using either potentiometric titration or fluorimetric detection. The experimental data for 1,8-ANS/beta-CD fit well to the 1:1 binding model, with an association constant of 87 +/- 1 M-1. The association constants of the 1:1 and 1:2 2,6-TNS/beta-CD complexes utilizing direct potentiometry were 3737 +/- 6 and 149 +/- 2 M-1. It is shown that fluorimetry can give biased estimates for the association constants of the complexation 2,6-TNS/beta-CD, since the assumption of an equivalent quantum yield of bound species is not valid.},
	keywords = {ANILINONAPHTHALENESULFONATES, COMPLEXATION, cyclodextrins, DOUBLE-RECIPROCAL PLOT, FLUORESCENCE, ion-selective electrodes, SCATCHARD PLOT},
	issn = {0724-8741},
	doi = {10.1023/A:1015808307322},
	author = {Sideris, EE and Valsami, GN and Koupparis, MA and Macheras, PE}
}
