<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sideris, EE</style></author><author><style face="normal" font="default" size="100%">Valsami, GN</style></author><author><style face="normal" font="default" size="100%">Koupparis, MA</style></author><author><style face="normal" font="default" size="100%">Macheras, PE</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Studies on the interaction of diflunisal ion with cyclodextrins using ion-selective electrode potentiometry</style></title><secondary-title><style face="normal" font="default" size="100%">EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">beta-cyclodextrin</style></keyword><keyword><style  face="normal" font="default" size="100%">binding</style></keyword><keyword><style  face="normal" font="default" size="100%">cooperative binding</style></keyword><keyword><style  face="normal" font="default" size="100%">diflunisal</style></keyword><keyword><style  face="normal" font="default" size="100%">gamma-cyclodextrin</style></keyword><keyword><style  face="normal" font="default" size="100%">Hill model</style></keyword><keyword><style  face="normal" font="default" size="100%">hydroxypropyl-beta-cyclodextrin</style></keyword><keyword><style  face="normal" font="default" size="100%">ion-selective electrodes</style></keyword><keyword><style  face="normal" font="default" size="100%">Scatchard model</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1999</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAR</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">4</style></number><publisher><style face="normal" font="default" size="100%">ELSEVIER SCIENCE BV</style></publisher><pub-location><style face="normal" font="default" size="100%">PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS</style></pub-location><volume><style face="normal" font="default" size="100%">7</style></volume><pages><style face="normal" font="default" size="100%">271-278</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The interaction of diflunisal ion (DF) with beta-cyclodextrin (PCD), gamma-cyclodextrin (gamma CD), and hydroxypropyl-beta-cyclodextrin (HP beta CD) was studied in phosphate buffer, pH 7.4, at 5-37 degrees C and various CD concentrations using a home-made diflunisal ion-selective electrode. Typical direct binding plots and Scatchard plots were obtained with HP beta CD. The Scatchard model for one class of binding sites was used for the estimation of binding parameters for the DF/HP beta CD interaction. The estimates for n (number of binding sites per CD molecule) were in all cases very close to unity, indicating 1:1 complexation. The association constant (K) estimates decrease with increasing temperature. Sigmoidal direct binding plots and concave-downwards Scatchard plots were obtained with various beta CD or gamma CD concentrations. The Hill model was used for the estimation of the binding parameters for the DF/beta CD and DF/gamma CD interactions. Both the Hill coefficients and the binding constants were markedly dependent on the CD concentration. These findings indicate the cooperative character of DF/beta CD and DF/gamma CD interactions. The foe energy change, Delta G, and the thermodynamic parameters, Delta H and Delta S, were estimated for each of the interactions studied using the Van't Hoff equation. (C) 1999 Published by Elsevier Science B.V. All rights reserved.</style></abstract><work-type><style face="normal" font="default" size="100%">Article</style></work-type></record></records></xml>