<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sideris, EE</style></author><author><style face="normal" font="default" size="100%">Georgiou, C.A.</style></author><author><style face="normal" font="default" size="100%">Koupparis, MA</style></author><author><style face="normal" font="default" size="100%">Macheras, PE</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">AUTOMATED FLOW-INJECTION SERIAL DYNAMIC DIALYSIS TECHNIQUE IN THE STUDY OF DRUG-BINDING WITH CYCLODEXTRINS</style></title><secondary-title><style face="normal" font="default" size="100%">ANALYTICA CHIMICA ACTA</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AUTOMATED SERIAL DYNAMIC DIALYSIS TECHNIQUE</style></keyword><keyword><style  face="normal" font="default" size="100%">cyclodextrins</style></keyword><keyword><style  face="normal" font="default" size="100%">DRUG BINDING TO CYCLODEXTRINS</style></keyword><keyword><style  face="normal" font="default" size="100%">FLOW INJECTION</style></keyword><keyword><style  face="normal" font="default" size="100%">Ibuprofen</style></keyword><keyword><style  face="normal" font="default" size="100%">P-NITROPHENOL</style></keyword><keyword><style  face="normal" font="default" size="100%">SALICYLIC ACID</style></keyword><keyword><style  face="normal" font="default" size="100%">Scatchard model</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1994</style></year><pub-dates><date><style  face="normal" font="default" size="100%">APR 20</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">1</style></number><publisher><style face="normal" font="default" size="100%">ELSEVIER SCIENCE BV</style></publisher><pub-location><style face="normal" font="default" size="100%">PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS</style></pub-location><volume><style face="normal" font="default" size="100%">289</style></volume><pages><style face="normal" font="default" size="100%">87-95</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A flow-injection dynamic dialysis technique is presented for the determination of binding parameters of drugs to cyclodextrins (CDs). The automated system consists of a flow-injection unit, the sample loop of which is the receiving compartment of a dialyser unit, and a home made timing module for operation control through two flow switching solenoid valves. The procedure of binding studies is rapid and yields reproducible results. Binding parameters of CD-micromolecule complexes was calculated using the Scatchard model. Typical examples of the binding of p-nitrophenol with alpha-CD(K-as=1.56x10(3) M(-1) at pH 7.4 and 2.06x10(3) M(-1) at pH 9.0), salicylic acid with beta-CD (K-as=3.8 x 10(2) M(-1) at pH 1.5 and 51 M(-1) at pH 7.4) and ibuprofen with beta-CD (K-as=2.2x10(2) M(-1) at pH 2.5) are presented and the binding constants obtained are compared to literature values. 1:1 stoichiometry was found in all cases and within run precision ranged from 2 to 14% R.S.D. The between run precision for the binding of p-nitrophenol to alpha-CD was 2% (n=3).</style></abstract><work-type><style face="normal" font="default" size="100%">Article</style></work-type></record></records></xml>