<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sideris, EE</style></author><author><style face="normal" font="default" size="100%">Valsami, GN</style></author><author><style face="normal" font="default" size="100%">Koupparis, MA</style></author><author><style face="normal" font="default" size="100%">Macheras, PE</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">DETERMINATION OF ASSOCIATION CONSTANTS IN CYCLODEXTRIN DRUG COMPLEXATION USING THE SCATCHARD PLOT - APPLICATION TO BETA-CYCLODEXTRIN-ANILINONAPHTHALENESULFONATES</style></title><secondary-title><style face="normal" font="default" size="100%">PHARMACEUTICAL RESEARCH</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ANILINONAPHTHALENESULFONATES</style></keyword><keyword><style  face="normal" font="default" size="100%">COMPLEXATION</style></keyword><keyword><style  face="normal" font="default" size="100%">cyclodextrins</style></keyword><keyword><style  face="normal" font="default" size="100%">DOUBLE-RECIPROCAL PLOT</style></keyword><keyword><style  face="normal" font="default" size="100%">FLUORESCENCE</style></keyword><keyword><style  face="normal" font="default" size="100%">ion-selective electrodes</style></keyword><keyword><style  face="normal" font="default" size="100%">SCATCHARD PLOT</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1992</style></year><pub-dates><date><style  face="normal" font="default" size="100%">DEC</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">12</style></number><publisher><style face="normal" font="default" size="100%">PLENUM PUBL CORP</style></publisher><pub-location><style face="normal" font="default" size="100%">233 SPRING ST, NEW YORK, NY 10013</style></pub-location><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">1568-1574</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The appropriate Scatchard equation was developed for a system involving the formation of 1:1 and 1:2 substrate:cyclodextrin complexes. Simulation of this system was performed under the most common experimental conditions encountered in this type of study. The use of the equation allows for nonlinear least-squares estimation of the association constants. The interaction of the model compounds 1-anilino-8-naphthalenesulfonate (1,8-ANS) and 2(p-toluidinyl)-6-naphthalenesulfonate (2,6-TNS) with beta-cyclodextrin (beta-CD) was used to evaluate the theoretical model. Binding experiments were performed using either potentiometric titration or fluorimetric detection. The experimental data for 1,8-ANS/beta-CD fit well to the 1:1 binding model, with an association constant of 87 +/- 1 M-1. The association constants of the 1:1 and 1:2 2,6-TNS/beta-CD complexes utilizing direct potentiometry were 3737 +/- 6 and 149 +/- 2 M-1. It is shown that fluorimetry can give biased estimates for the association constants of the complexation 2,6-TNS/beta-CD, since the assumption of an equivalent quantum yield of bound species is not valid.</style></abstract><work-type><style face="normal" font="default" size="100%">Article</style></work-type></record></records></xml>