<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Valsami, GN</style></author><author><style face="normal" font="default" size="100%">Macheras, PE</style></author><author><style face="normal" font="default" size="100%">Koupparis, MA</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">BINDING STUDY OF THE FLUORESCENCE PROBE 1-ANILINO-8-NAPHTHALENESULFONATE TO HUMAN PLASMA AND HUMAN AND BOVINE SERUM-ALBUMIN USING POTENTIOMETRIC TITRATION</style></title><secondary-title><style face="normal" font="default" size="100%">PHARMACEUTICAL RESEARCH</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">1-ANILINO-8-NAPHTHALENESULFONATE (ANS)</style></keyword><keyword><style  face="normal" font="default" size="100%">BOVINE SERUM ALBUMIN</style></keyword><keyword><style  face="normal" font="default" size="100%">HUMAN SERUM ALBUMIN</style></keyword><keyword><style  face="normal" font="default" size="100%">ion selective electrodes</style></keyword><keyword><style  face="normal" font="default" size="100%">PLASMA BINDING</style></keyword><keyword><style  face="normal" font="default" size="100%">POTENTIOMETRIC TITRATION</style></keyword><keyword><style  face="normal" font="default" size="100%">Protein Binding</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1991</style></year><pub-dates><date><style  face="normal" font="default" size="100%">JUL</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">7</style></number><publisher><style face="normal" font="default" size="100%">PLENUM PUBL CORP</style></publisher><pub-location><style face="normal" font="default" size="100%">233 SPRING ST, NEW YORK, NY 10013</style></pub-location><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">888-892</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The binding of 1-anilino-8-naphthalenesulfonate (ANS) to bovine serum albumin (BSA), human serum albumin (HSA), and human plasma has been studied by potentiometric titration utilizing a laboratory constructed ion selective electrode (ISE) of ANS. Three classes of ANS binding sites were found on BSA, HSA, and plasma at 25 and 37-degrees-C. Computer analysis of the data resulted in estimates for the association constants, number of binding sites (HSA, BSA), and binding capacity of each class. The association constants for the first class of binding sites at 25-degrees-C were found to be 7.53 (+/- 0.59) x 10(5), 2.70 (+/- 0.20) x 10(5), and 2.64 (+/- 0.26) x 10(5) M-1 for BSA, HSA, and plasma, respectively. Lower values for the association constants of all binding classes were estimated at the higher temperature (37-degrees-C). The binding capacity for ANS decreased in the order BSA, plasma, HSA.</style></abstract><work-type><style face="normal" font="default" size="100%">Article</style></work-type></record></records></xml>