@article {10178,
	title = {Validation of a real-time quantitative polymerase chain reaction method for the quantification of 3 survivin transcripts and evaluation in breast cancer tissues.},
	journal = {Clin Breast Cancer},
	volume = {14},
	year = {2014},
	month = {2014 Apr},
	pages = {122-31},
	abstract = {BACKGROUND: Survivin is a novel antiapoptotic gene, which is a member of the inhibitor of apoptosis protein (IAP) family. Recently, 3 splice variants of this gene were cloned and characterized. This study aimed to validate a sensitive and specific method for the detection of survivin variants in breast cancer.

METHODS: Real-time quantitative polymerase chain reaction (qPCR) was performed on the cDNA with a reverse primer specific for each splice variant and a pair of common hybridization probes.

RESULTS: The expression of wild-type survivin was significantly correlated with survivin-2b, survivin-ΔEx3, and the ratio of survivin-ΔEx3 to wild-type survivin (P < .001). The ratio of survivin-2b to wild-type survivin was strongly associated with the ratio of survivin-ΔEx3 to wild-type survivin (P < .001). There was a strong positive association between the grade of the tumor and survivin-2b mRNA, survivin-ΔEx3 mRNA, and the ratio of survivin-ΔEx3 to wild-type survivin mRNA (P < .05). The ratio of survivin-2b to wild-type survivin was significantly associated with the presence of estrogen receptors (P = .05).

CONCLUSION: Our validated data suggest that survivin isoforms may be related to clinicopathological features and could be used as molecular prognostic tools or as new therapy targets.},
	keywords = {Breast Neoplasms, Female, Gene Expression Regulation, Neoplastic, Humans, Inhibitor of Apoptosis Proteins, Neoplasm Grading, Neoplasm Invasiveness, Prognosis, Protein Isoforms, RNA Splicing, Survival Rate},
	issn = {1938-0666},
	doi = {10.1016/j.clbc.2013.10.012},
	author = {Pavlidou, Anastasia and Kroupis, Christos and Goutas, Nikolaos and Dalamaga, Maria and Dimas, Kleanthi}
}
