@article {27055,
	title = {miR-221/222 cluster expression improves clinical stratification of non-muscle invasive bladder cancer (TaT1) patients{\textquoteright} risk for short-term relapse and progression},
	journal = {Genes Chromosomes Cancer},
	volume = {57},
	number = {3},
	year = {2018},
	note = {Tsikrika, Foteini DAvgeris, MargaritisLevis, Panagiotis KTokas, TheodorosStravodimos, KonstantinosScorilas, Andreaseng2017/11/29 06:00Genes Chromosomes Cancer. 2018 Mar;57(3):150-161. doi: 10.1002/gcc.22516. Epub 2017 Dec 15.},
	month = {Mar},
	pages = {150-161},
	abstract = {Clinical heterogeneity of bladder cancer prognosis requires the identification of bladder tumors{\textquoteright} molecular profile to improve the prediction value of the established and clinically used markers. In this study, we have analyzed miR-221/222 cluster expression in bladder tumors and its clinical significance for patients{\textquoteright} prognosis and disease outcome. The study included 387 tissue specimens. Following extraction, total RNA was polyadenylated at 3{\textquoteright}-end and reversed transcribed. SYBR-Green based qPCR assays were performed for the quantification of miR-221/222 expression. Extensive statistical analysis was completed for the evaluation of miR-221/222 cluster{\textquoteright}s clinical significance. The expression of miR-221/222 is significantly downregulated in tumors compared to normal urothelium, while ROC curve and logistic regression analysis highlighted cluster{\textquoteright}s discriminatory ability. However, miR-222 levels were increased in muscle-invasive (T2-T4) compared to superficial tumors (TaT1), and in high compared to low-grade tumors. Kaplan-Meier survival curves and Cox regression analysis revealed the stronger risk of TaT1 patients overexpressing miR-222 for disease short-term relapse and progression following treatment. Moreover, multivariate Cox models highlighted the independent prognostic value of miR-222 overexpression for TaT1 patients{\textquoteright} poor prognosis. Finally, the analysis of miR-222 expression improved significantly the positive prediction strength of the clinically used prognostic markers of tumor stage, grade, EORTC risk-stratification and recurrence at the first follow-up cystoscopy for TaT1 patients{\textquoteright} outcome, and resulted to higher clinical net benefit following decision curve analysis. In conclusion, the expression of miR-221/222 cluster is deregulated in bladder tumors and miR-222 overexpression results to a superior positive prediction of TaT1 patients{\textquoteright} short-term relapse and progression.},
	keywords = {Aged, Aged, 80 and over, Biomarkers, Tumor/genetics, Disease Progression, Female, Genetic Predisposition to Disease, Humans, Male, MicroRNAs/biosynthesis/*genetics, Neoplasm Recurrence, Local/genetics/pathology, Neoplasm Staging, Prognosis, Urinary Bladder Neoplasms/*genetics/pathology},
	isbn = {1098-2264 (Electronic)1045-2257 (Linking)},
	author = {Tsikrika, F. D. and Avgeris, M. and Levis, P. K. and Tokas, T. and Stravodimos, K. and Scorilas, A.}
}
