@article {31423,
	title = {A Cancer-Related microRNA Signature Shows Biomarker Utility in Multiple Myeloma},
	journal = {Int J Mol Sci},
	volume = {22},
	year = {2021},
	abstract = {Multiple myeloma (MM) is the second most common hematological malignancy, arising from terminally differentiated B cells, namely plasma cells. miRNAs are small non-coding RNAs that participate in the post-transcriptional regulation of gene expression. In this study, we investigated the role of nine miRNAs in MM. CD138+ plasma cells were selected from bone marrow aspirates from MM and smoldering MM (sMM) patients. Total RNA was extracted and in vitro polyadenylated. Next, first-strand cDNA synthesis was performed using an oligo-dT-adapter primer. For the relative quantification of the investigated miRNAs, an in-house real-time quantitative PCR (qPCR) assay was developed. A functional in silico analysis of the miRNAs was also performed. miR-16-5p and miR-155-5p expression was significantly lower in the CD138+ plasma cells of MM patients than in those of sMM patients. Furthermore, lower levels of miR-15a-5p, miR-16-5p, and miR-222-3p were observed in the CD138+ plasma cells of MM patients with osteolytic bone lesions, compared to those without. miR-125b-5p was also overexpressed in the CD138+ plasma cells of MM patients with bone disease that presented with skeletal-related events (SREs). Furthermore, lower levels of miR-223-3p were associated with significantly worse overall survival in MM patients. In conclusion, we propose a miRNA signature with putative clinical utility in MM.},
	author = {Papanota, A. M. and Karousi, P and Kontos, C.K. and Artemaki, P.I. and Liacos, C. I. and Papadimitriou, M. A. and Bagratuni, T. and Eleutherakis-Papaiakovou, E. and Malandrakis, P. and Ntanasis-Stathopoulos, I. and Gavriatopoulou, M. and Kastritis, E. and Avgeris, M. and Dimopoulos, M.A. and Scorilas, A. and Terpos, E.}
}
