@article {44701,
	title = {Delta SARS-CoV-2 variant is entirely substituted by the omicron variant during the fifth COVID-19 wave in Attica region},
	journal = {Sci Total Environ},
	volume = {856},
	number = {Pt 1},
	year = {2023},
	note = {Galani, AikateriniMarkou, AthinaDimitrakopoulos, LamprosKontou, AikateriniKostakis, MariosKapes, VasileiosDiamantopoulos, Marios AAdamopoulos, Panagiotis GAvgeris, MargaritisLianidou, EviScorilas, AndreasParaskevis, DimitriosTsiodras, SotiriosDimopoulos, Meletios-AthanasiosThomaidis, NikolaosengNetherlands2022/10/02 06:00Sci Total Environ. 2023 Jan 15;856(Pt 1):159062. doi: 10.1016/j.scitotenv.2022.159062. Epub 2022 Sep 29.},
	month = {Jan 15},
	pages = {159062},
	abstract = {Wastewater analysis is the most attractive alternative way for the quantification and variant profiling of SARS-CoV-2. Infection dynamics can be monitored by RT-qPCR assays while NGS can provide evidence for the presence of existing or new emerging SARS-CoV-2 variants. Herein, apart from the infection dynamic in Attica since June 1st, 2021, the monitoring of 9 mutations of the omicron and 4 mutations of the delta SARS-CoV-2 variants, utilizing both novel Nested-Seq and RT-PCR, is reported and the substitution of the delta variant (B.1.617.2) by the omicron variant (B.1.1.529) in Attica, Greece within approximately one month is highlighted. The key difference between the two methodologies is discovery power. RT-PCR can only detect known sequences cost-effectively, while NGS is a hypothesis-free approach that does not require prior knowledge to detect novel genes. Overall, the potential of wastewater genomic surveillance for the early discovery and monitoring of variants important for disease management at the community level is underlined. This is the first study, reporting the SARS-CoV-2 infection dynamic for an extended time period and the first attempt to monitor two of the most severe variants with two different methodologies in Greece.},
	keywords = {*Covid-19, *SARS-CoV-2/genetics, competing financial interests or personal relationships that could have appeared, delta variant, Greece, Humans, Ngs, Omicron variant, RT-qPCR, SARS-CoV-2, to influence the work reported in this paper., Wastewater},
	isbn = {1879-1026 (Electronic)0048-9697 (Print)0048-9697 (Linking)},
	author = {Galani, A. and Markou,A. and Dimitrakopoulos, L and Kontou, A. and Kostakis, M. and Kapes, V and Diamantopoulos, M.A. and Adamopoulos, P.G. and Avgeris, M. and Lianidou,E. and Scorilas, A. and Paraskevis, D and Tsiodras,S. and Dimopoulos, M.A. and Thomaidis, N.}
}
