@article {44715,
	title = {Generation of Non-Small Cell Lung Cancer Patient-Derived Xenografts to Study Intratumor Heterogeneity},
	journal = {Cancers},
	volume = {13},
	number = {10},
	year = {2021},
	note = {Kanaki, ZoiVoutsina, AlexandraMarkou, AthinaPateras, Ioannis SPotaris, KonstantinosAvgeris, MargaritisMakrythanasis, PeriklisAthanasiadis, Emmanouil IVamvakaris, IoannisPatsea, EleniVachlas, KonstantinosLianidou, EviGeorgoulias, VassilisKotsakis, AthanasiosKlinakis, Apostoloseng1964/Hellenic Foundation for Research and Innovation/801347/Horizon 2020 Framework Programme/Switzerland2021/06/03 06:00Cancers (Basel). 2021 May 18;13(10):2446. doi: 10.3390/cancers13102446.},
	month = {May 18},
	abstract = {Recent advances in sequencing technologies have allowed the in-depth molecular study of tumors, even at the single cell level. Sequencing efforts have uncovered a previously unappreciated heterogeneity among tumor cells, which has been postulated to be the driving force of tumor evolution and to facilitate recurrence, metastasis, and drug resistance. In the current study, focused on early-stage operable non-small cell lung cancer, we used tumor growth in patient-derived xenograft (PDX) models in mice as a fast-forward tumor evolution process to investigate the molecular characteristics of tumor cells that grow in mice, as well as the parameters that affect the grafting efficiency. We found that squamous cell carcinomas grafted significantly more efficiently compared with adenocarcinomas. Advanced stage, patient age and primary tumor size were positively correlated with grafting. Additionally, we isolated and characterized circulating tumor cells (CTC) from patients{\textquoteright} peripheral blood and found that the presence of CTCs expressing epithelial-to-mesenchymal (EMT) markers correlated with the grafting potential. Interestingly, exome sequencing of the PDX tumor identified genetic alterations in DNA repair and genome integrity genes that were under-represented in the human primary counterpart. In conclusion, through the generation of a PDX biobank of NSCLC, we identified the clinical and molecular properties of tumors that affected growth in mice.},
	keywords = {genetic profiling, intratumor heterogeneity, Non-small cell lung cancer, patient-derived xenografts},
	isbn = {2072-6694 (Print)2072-6694 (Electronic)2072-6694 (Linking)},
	author = {Kanaki, Z. and Voutsina, A. and Markou,A. and Pateras,I.S. and Potaris, K. and Avgeris, M. and Makrythanasis, P. and Athanasiadis, E. I. and Vamvakaris, I. and Patsea, E. and Vachlas, K. and Lianidou,E. and Georgoulias, V. and Kotsakis, A. and Klinakis, A}
}
