<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Christodoulou, M. I.</style></author><author><style face="normal" font="default" size="100%">Avgeris, M.</style></author><author><style face="normal" font="default" size="100%">Kokkinopoulou, I.</style></author><author><style face="normal" font="default" size="100%">Maratou, E.</style></author><author><style face="normal" font="default" size="100%">Mitrou, P.</style></author><author><style face="normal" font="default" size="100%">Kontos, C.K.</style></author><author><style face="normal" font="default" size="100%">Pappas, E.</style></author><author><style face="normal" font="default" size="100%">Boutati, E.</style></author><author><style face="normal" font="default" size="100%">Scorilas, A.</style></author><author><style face="normal" font="default" size="100%">Fragoulis, E.G.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Blood-based analysis of type-2 diabetes mellitus susceptibility genes identifies specific transcript variants with deregulated expression and association with disease risk</style></title><secondary-title><style face="normal" font="default" size="100%">Sci Rep</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*Genetic Predisposition to Disease</style></keyword><keyword><style  face="normal" font="default" size="100%">*Polymorphism, Single Nucleotide</style></keyword><keyword><style  face="normal" font="default" size="100%">Adult</style></keyword><keyword><style  face="normal" font="default" size="100%">Aged</style></keyword><keyword><style  face="normal" font="default" size="100%">Biomarkers/*blood</style></keyword><keyword><style  face="normal" font="default" size="100%">Case-Control Studies</style></keyword><keyword><style  face="normal" font="default" size="100%">Diabetes Mellitus, Type 2/blood/*genetics/*pathology</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">Follow-Up Studies</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression Regulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Genotype</style></keyword><keyword><style  face="normal" font="default" size="100%">High-Throughput Nucleotide Sequencing/*methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Leukocytes, Mononuclear/*metabolism/pathology</style></keyword><keyword><style  face="normal" font="default" size="100%">Male</style></keyword><keyword><style  face="normal" font="default" size="100%">Middle Aged</style></keyword><keyword><style  face="normal" font="default" size="100%">Prognosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Young Adult</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Feb 6</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">1</style></number><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">1512</style></pages><isbn><style face="normal" font="default" size="100%">2045-2322 (Electronic)2045-2322 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Despite significant progress by genome-wide association studies, the ability of genetic variants to conduce to the prediction or prognosis of type-2 diabetes (T2D) is weak. Expression analysis of the corresponding genes may suggest possible links between single-nucleotide polymorphisms and T2D phenotype and/or risk. Herein, we investigated the expression patterns of 24 T2D-susceptibility genes, and their individual transcript variants (tv), in peripheral blood of T2D patients and controls (CTs), applying RNA-seq and real-time qPCR methodologies, and explore possible associations with disease features. Our data revealed the deregulation of certain transcripts in T2D patients. Among them, the down-regulation of CAPN10 tv3 was confirmed as an independent predictor for T2D. In patients, increased expression of CDK5 tv2, CDKN2A tv3 or THADA tv5 correlated positively with serum insulin levels, of CDK5 tv1 positively with % HbA1c levels, while in controls, elevated levels of TSPAN8 were associated positively with the presence of T2D family history. Herein, a T2D-specific expression profile of specific transcripts of disease-susceptibility genes is for the first time described in human peripheral blood. Large-scale studies are needed to evaluate the potential of these molecules to serve as disease biomarkers.</style></abstract><accession-num><style face="normal" font="default" size="100%">30728419</style></accession-num><notes><style face="normal" font="default" size="100%">Christodoulou, Maria-IoannaAvgeris, MargaritisKokkinopoulou, IoannaMaratou, EiriniMitrou, PanayotaKontos, Christos KPappas, EfthimiosBoutati, EleniScorilas, AndreasFragoulis, Emmanuel GengResearch Support, Non-U.S. Gov'tEngland2019/02/08 06:00Sci Rep. 2019 Feb 6;9(1):1512. doi: 10.1038/s41598-018-37856-1.</style></notes><custom2><style face="normal" font="default" size="100%">PMC6365563</style></custom2><auth-address><style face="normal" font="default" size="100%">Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece. mchrist@biol.uoa.gr.Hellenic National Center for Research, Prevention and Treatment of Diabetes Mellitus and its Complications (HNDC), Athens, Greece. mchrist@biol.uoa.gr.Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, UK. mchrist@biol.uoa.gr.Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece.Hellenic National Center for Research, Prevention and Treatment of Diabetes Mellitus and its Complications (HNDC), Athens, Greece.Second Department of Internal Medicine, School of Medicine, Attikon Hospital, National and Kapodistrian University of Athens, Athens, Greece.</style></auth-address></record></records></xml>