<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Foutadakis, S.</style></author><author><style face="normal" font="default" size="100%">Soureas, K.</style></author><author><style face="normal" font="default" size="100%">Roupakia, E.</style></author><author><style face="normal" font="default" size="100%">Besta, S.</style></author><author><style face="normal" font="default" size="100%">Avgeris, M.</style></author><author><style face="normal" font="default" size="100%">Kolettas, E.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Identification of Oncogene-Induced Senescence-Associated MicroRNAs</style></title><secondary-title><style face="normal" font="default" size="100%">Methods Mol Biol</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*Cellular Senescence/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">*MicroRNAs/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">*Oncogenes/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Bioinformatics</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression Profiling/methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression Regulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice</style></keyword><keyword><style  face="normal" font="default" size="100%">microRNAs</style></keyword><keyword><style  face="normal" font="default" size="100%">miRNA-seq</style></keyword><keyword><style  face="normal" font="default" size="100%">miRNA-sequencing</style></keyword><keyword><style  face="normal" font="default" size="100%">Nanostring miRNA analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Oncogene-induced senescence</style></keyword><keyword><style  face="normal" font="default" size="100%">Real-Time Polymerase Chain Reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">RT-qPCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Small RNA-seq</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year></dates><volume><style face="normal" font="default" size="100%">2906</style></volume><pages><style face="normal" font="default" size="100%">189-213</style></pages><isbn><style face="normal" font="default" size="100%">1940-6029 (Electronic)1064-3745 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Cellular senescence, a state of permanent cell cycle arrest, recapitulates the aging process at the cellular level. It can be triggered by intrinsic or extrinsic factors including telomere shortening (replicative senescence) and in response to various types of stresses such as oncogenic stress (oncogene-induced senescence, OIS). Senescence has been detected in vitro and in premalignant lesions in mice and humans expressing mutant oncogenes. MicroRNAs (miRNAs) are short noncoding RNAs that regulate gene expression at the posttranscriptional level, and have been involved in both replicative senescence and OIS. Several methods have been used to identify miRNAs and compare their expression in normal versus oncogene-induced senescent cells, as well as to analyze their role and their targets in senescence. Here, we describe several methods that can be employed to identify miRNAs in cells undergoing OIS, including miRNA-sequencing, RT-qPCR-based detection and quantification of miRNAs and Nanostring miRNA analysis (nCounter miRNA Expression Assay). Moreover, we perform a meta-analysis of studies employing the above methodologies, pinpoint miRNAs with consistent expression changes across senescence models, and predict their target genes and the pathways in which they partake.</style></abstract><accession-num><style face="normal" font="default" size="100%">40082357</style></accession-num><notes><style face="normal" font="default" size="100%">Foutadakis, SpyrosSoureas, KonstantinosRoupakia, EugeniaBesta, SimoniAvgeris, MargaritisKolettas, Evangeloseng2025/03/14 11:17Methods Mol Biol. 2025;2906:189-213. doi: 10.1007/978-1-0716-4426-3_11.</style></notes><auth-address><style face="normal" font="default" size="100%">Center of Basic Research, Biomedical Research Foundation, Academy of Athens, Athens, Greece.Hellenic Institute for the Study of Sepsis, Athens, Greece.Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece.Laboratory of Clinical Biochemistry-Molecular Diagnostics, Second Department of Pediatrics, School of Medicine, National and Kapodistrian University of Athens, 'P. &amp; A. Kyriakou' Children's Hospital, Athens, Greece.Laboratory of Biology, School of Medicine, Faculty of Health Sciences, and Institute of Biosciences, Centre for Research and Innovation, University of Ioannina, Ioannina, Greece.Molecular Cancer Biology &amp; Senescence Group, Biomedical Research Institute, Foundation for Research and Technology, Ioannina, Greece.International Oncology Institute, The first affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310053, China.Laboratory of Biology, School of Medicine, Faculty of Health Sciences, and Institute of Biosciences, Centre for Research and Innovation, University of Ioannina, Ioannina, Greece. ekoletas@uoi.gr.Molecular Cancer Biology &amp; Senescence Group, Biomedical Research Institute, Foundation for Research and Technology, Ioannina, Greece. ekoletas@uoi.gr.</style></auth-address></record></records></xml>