<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Galani, A.</style></author><author><style face="normal" font="default" size="100%">Aalizadeh, R.</style></author><author><style face="normal" font="default" size="100%">Kostakis, M.</style></author><author><style face="normal" font="default" size="100%">Markou,A.</style></author><author><style face="normal" font="default" size="100%">Alygizakis, N.</style></author><author><style face="normal" font="default" size="100%">Lytras, T.</style></author><author><style face="normal" font="default" size="100%">Adamopoulos, P.G.</style></author><author><style face="normal" font="default" size="100%">Peccia, J.</style></author><author><style face="normal" font="default" size="100%">Thompson, D. C.</style></author><author><style face="normal" font="default" size="100%">Kontou, A.</style></author><author><style face="normal" font="default" size="100%">Karagiannidis, A.</style></author><author><style face="normal" font="default" size="100%">Lianidou,E.S.</style></author><author><style face="normal" font="default" size="100%">Avgeris, M.</style></author><author><style face="normal" font="default" size="100%">Paraskevis, D</style></author><author><style face="normal" font="default" size="100%">Tsiodras,S.</style></author><author><style face="normal" font="default" size="100%">Scorilas, A.</style></author><author><style face="normal" font="default" size="100%">Vasiliou, V.</style></author><author><style face="normal" font="default" size="100%">Dimopoulos, M.A.</style></author><author><style face="normal" font="default" size="100%">Thomaidis, N.S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">SARS-CoV-2 wastewater surveillance data can predict hospitalizations and ICU admissions</style></title><secondary-title><style face="normal" font="default" size="100%">Sci Total Environ</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">(Case Number: 231-0004089064) and on March 22, 2021 to the Patent Cooperation</style></keyword><keyword><style  face="normal" font="default" size="100%">*Covid-19</style></keyword><keyword><style  face="normal" font="default" size="100%">*SARS-CoV-2</style></keyword><keyword><style  face="normal" font="default" size="100%">Andreas Scorilas, Margaritis Avgeris, Panagiotis G. Adamopoulos and Nikolaos S.</style></keyword><keyword><style  face="normal" font="default" size="100%">competing interests.</style></keyword><keyword><style  face="normal" font="default" size="100%">Covid-19</style></keyword><keyword><style  face="normal" font="default" size="100%">Detection and mutational analysis of an RNA virus in an environmental sample</style></keyword><keyword><style  face="normal" font="default" size="100%">Hospital admission rates</style></keyword><keyword><style  face="normal" font="default" size="100%">Hospitalization</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">ICU admission rates</style></keyword><keyword><style  face="normal" font="default" size="100%">Intensive Care Units</style></keyword><keyword><style  face="normal" font="default" size="100%">Method validation</style></keyword><keyword><style  face="normal" font="default" size="100%">RNA, Viral</style></keyword><keyword><style  face="normal" font="default" size="100%">RT-qPCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Thomaidis on February 11, 2021 to the Hellenic Industrial Property Organisation</style></keyword><keyword><style  face="normal" font="default" size="100%">Treaty (PCT application number: PCT/EP2021/057316). All other authors declare no</style></keyword><keyword><style  face="normal" font="default" size="100%">Wastewater</style></keyword><keyword><style  face="normal" font="default" size="100%">Wastewater-Based Epidemiological Monitoring</style></keyword><keyword><style  face="normal" font="default" size="100%">wastewater-based epidemiology</style></keyword><keyword><style  face="normal" font="default" size="100%">were filed by the National and Kapodistrian University of Athens, and the authors</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan 15</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">804</style></volume><pages><style face="normal" font="default" size="100%">150151</style></pages><isbn><style face="normal" font="default" size="100%">1879-1026 (Electronic)0048-9697 (Print)0048-9697 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">We measured SARS-CoV-2 RNA load in raw wastewater in Attica, Greece, by RT-qPCR for the environmental surveillance of COVID-19 for 6 months. The lag between RNA load and pandemic indicators (COVID-19 hospital and intensive care unit (ICU) admissions) was calculated using a grid search. Our results showed that RNA load in raw wastewater is a leading indicator of positive COVID-19 cases, new hospitalization and admission into ICUs by 5, 8 and 9 days, respectively. Modelling techniques based on distributed/fixed lag modelling, linear regression and artificial neural networks were utilized to build relationships between SARS-CoV-2 RNA load in wastewater and pandemic health indicators. SARS-CoV-2 mutation analysis in wastewater during the third pandemic wave revealed that the alpha-variant was dominant. Our results demonstrate that clinical and environmental surveillance data can be combined to create robust models to study the on-going COVID-19 infection dynamics and provide an early warning for increased hospital admissions.</style></abstract><accession-num><style face="normal" font="default" size="100%">34623953</style></accession-num><notes><style face="normal" font="default" size="100%">Galani, AikateriniAalizadeh, RezaKostakis, MariosMarkou, AthinaAlygizakis, NikiforosLytras, TheodoreAdamopoulos, Panagiotis GPeccia, JordanThompson, David CKontou, AikateriniKaragiannidis, ApostolosLianidou, Evi SAvgeris, MargaritisParaskevis, DimitriosTsiodras, SotiriosScorilas, AndreasVasiliou, VasilisDimopoulos, Meletios-AthanasiosThomaidis, Nikolaos SengUL1 TR001863/TR/NCATS NIH HHS/Netherlands2021/10/09 06:00Sci Total Environ. 2022 Jan 15;804:150151. doi: 10.1016/j.scitotenv.2021.150151. Epub 2021 Sep 7.</style></notes><custom2><style face="normal" font="default" size="100%">PMC8421077</style></custom2><auth-address><style face="normal" font="default" size="100%">Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, University Campus, Zografou, 15771, Athens, Greece.School of Medicine, European University Cyprus, Cyprus.Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece.Department of Chemical and Environmental Engineering, School of Engineering and Applied Science, Yale University, New Haven, CT, USA.Department of Clinical Pharmacy, School of Pharmacy and Pharmaceutical Sciences, University of Colorado Skaggs, Aurora, CO, USA; Department of Environmental Health Sciences, Yale School of Public Health, Yale University, New Haven, CT 06510, USA.Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece; Laboratory of Clinical Biochemistry - Molecular Diagnostics, Second Department of Pediatrics, &quot;P. &amp; A. Kyriakou&quot; Children's Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.Department of Hygiene Epidemiology and Medical Statistics, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece.Fourth Department of Internal Medicine, School of Medicine, University General Hospital Attikon, National and Kapodistrian University of Athens, Greece.Department of Environmental Health Sciences, Yale School of Public Health, Yale University, New Haven, CT 06510, USA.Department of Clinical Therapeutics, School of Medicine, National and Kapodistrian University of Athens, Greece.Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, University Campus, Zografou, 15771, Athens, Greece. Electronic address: ntho@chem.uoa.gr.</style></auth-address></record></records></xml>