<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alygizakis, N.</style></author><author><style face="normal" font="default" size="100%">Markou, A. N.</style></author><author><style face="normal" font="default" size="100%">Rousis, N. I.</style></author><author><style face="normal" font="default" size="100%">Galani, A.</style></author><author><style face="normal" font="default" size="100%">Avgeris, M.</style></author><author><style face="normal" font="default" size="100%">Adamopoulos, P.G.</style></author><author><style face="normal" font="default" size="100%">Scorilas, A.</style></author><author><style face="normal" font="default" size="100%">Lianidou,E.S.</style></author><author><style face="normal" font="default" size="100%">Paraskevis, D</style></author><author><style face="normal" font="default" size="100%">Tsiodras,S.</style></author><author><style face="normal" font="default" size="100%">Tsakris, A.</style></author><author><style face="normal" font="default" size="100%">Dimopoulos, M.A.</style></author><author><style face="normal" font="default" size="100%">Thomaidis, N.S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Analytical methodologies for the detection of SARS-CoV-2 in wastewater: Protocols and future perspectives</style></title><secondary-title><style face="normal" font="default" size="100%">Trends in Analytical Chemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">concentration</style></keyword><keyword><style  face="normal" font="default" size="100%">COVID-19 pandemic</style></keyword><keyword><style  face="normal" font="default" size="100%">in this paper.</style></keyword><keyword><style  face="normal" font="default" size="100%">influent wastewater</style></keyword><keyword><style  face="normal" font="default" size="100%">isolation</style></keyword><keyword><style  face="normal" font="default" size="100%">molecular methods detection</style></keyword><keyword><style  face="normal" font="default" size="100%">personal relationships that could have appeared to influence the work presented</style></keyword><keyword><style  face="normal" font="default" size="100%">RT-qPCR</style></keyword><keyword><style  face="normal" font="default" size="100%">sampling</style></keyword><keyword><style  face="normal" font="default" size="100%">symptomatic and asymptomatic</style></keyword><keyword><style  face="normal" font="default" size="100%">temperature storage</style></keyword><keyword><style  face="normal" font="default" size="100%">wastewater-based epidemiology</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">134</style></volume><pages><style face="normal" font="default" size="100%">116125</style></pages><isbn><style face="normal" font="default" size="100%">0165-9936 (Print)1879-3142 (Electronic)0165-9936 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In March 2020 the World Health Organization announced a pandemic outbreak. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative pathogen for the coronavirus disease-19 (COVID-19) pandemic. The authorities worldwide use clinical science to identify infected people, but this approach is not able to track all symptomatic and asymptomatic cases due to limited sampling capacity of the testing laboratories. This drawback is eliminated by the Wastewater-Based Epidemiology (WBE) approach. In this review, we summarized the peer-reviewed published literature (available as of September 28, 2020), in the field of WBE. The commonly used steps (sampling, storage, concentration, isolation, detection) of the analytical protocols were identified. The potential limitations of each stage of the protocols and good practices were discussed. Finally, new methods for the efficient detection of SARS-CoV-2 were proposed.</style></abstract><accession-num><style face="normal" font="default" size="100%">33235400</style></accession-num><notes><style face="normal" font="default" size="100%">Alygizakis, NikiforosMarkou, Athina NRousis, Nikolaos IGalani, AikateriniAvgeris, MargaritisAdamopoulos, Panagiotis GScorilas, AndreasLianidou, Evi SParaskevis, DimitriosTsiodras, SotiriosTsakris, AthanassiosDimopoulos, Meletios-AthanasiosThomaidis, Nikolaos SengReviewNetherlands2020/11/26 06:00Trends Analyt Chem. 2021 Jan;134:116125. doi: 10.1016/j.trac.2020.116125. Epub 2020 Nov 20.</style></notes><custom2><style face="normal" font="default" size="100%">PMC7677696</style></custom2><auth-address><style face="normal" font="default" size="100%">Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Greece.Laboratory of Clinical Biochemistry and Molecular Diagnostics, Second Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, Athens, Greece.Laboratory of Biochemistry and Molecular Biology, Department of Biology, National and Kapodistrian University of Athens, Greece.Department of Hygiene Epidemiology and Medical Statistics, School of Medicine, National and Kapodistrian University of Athens, Greece.Fourth Department of Propedeutic Internal Medicine, School of Medicine, University General Hospital Attikon, National and Kapodistrian University of Athens, Greece.Department of Microbiology, School of Medicine, National and Kapodistrian University of Athens, Greece.Department of Clinical Therapeutics, Medical School, National and Kapodistrian University of Athens, Greece.</style></auth-address></record></records></xml>