<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Avgeris, M.</style></author><author><style face="normal" font="default" size="100%">Stamati, L.</style></author><author><style face="normal" font="default" size="100%">Kontos, C.K.</style></author><author><style face="normal" font="default" size="100%">Piatopoulou, D.</style></author><author><style face="normal" font="default" size="100%">Marmarinos, A.</style></author><author><style face="normal" font="default" size="100%">Xagorari, M.</style></author><author><style face="normal" font="default" size="100%">Baka, M.</style></author><author><style face="normal" font="default" size="100%">Doganis, D.</style></author><author><style face="normal" font="default" size="100%">Anastasiou, T.</style></author><author><style face="normal" font="default" size="100%">Kosmidis, H.</style></author><author><style face="normal" font="default" size="100%">Gourgiotis, D.</style></author><author><style face="normal" font="default" size="100%">Scorilas, A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">BCL2L12 improves risk stratification and prediction of BFM-chemotherapy response in childhood acute lymphoblastic leukemia</style></title><secondary-title><style face="normal" font="default" size="100%">Clin Chem Lab Med</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antineoplastic Combined Chemotherapy Protocols/*pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis/drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Bcl2</style></keyword><keyword><style  face="normal" font="default" size="100%">BCL2-like 12</style></keyword><keyword><style  face="normal" font="default" size="100%">Berlin-Frankfurt-Munster</style></keyword><keyword><style  face="normal" font="default" size="100%">Biomarker</style></keyword><keyword><style  face="normal" font="default" size="100%">cancer marker</style></keyword><keyword><style  face="normal" font="default" size="100%">Child</style></keyword><keyword><style  face="normal" font="default" size="100%">Child, Preschool</style></keyword><keyword><style  face="normal" font="default" size="100%">Childhood ALL</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">hematological cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunophenotyping</style></keyword><keyword><style  face="normal" font="default" size="100%">Infant</style></keyword><keyword><style  face="normal" font="default" size="100%">Male</style></keyword><keyword><style  face="normal" font="default" size="100%">Muscle Proteins/*genetics/immunology</style></keyword><keyword><style  face="normal" font="default" size="100%">Neoplasm, Residual</style></keyword><keyword><style  face="normal" font="default" size="100%">pediatric cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">polymerase chain reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">Precursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis/*drug therapy/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Proto-Oncogene Proteins c-bcl-2/*genetics/immunology</style></keyword><keyword><style  face="normal" font="default" size="100%">Risk Factors</style></keyword><keyword><style  face="normal" font="default" size="100%">RNA, Neoplasm/genetics/immunology/isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">tumor marker</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Nov 27</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">12</style></number><volume><style face="normal" font="default" size="100%">56</style></volume><pages><style face="normal" font="default" size="100%">2104-2118</style></pages><isbn><style face="normal" font="default" size="100%">1437-4331 (Electronic)1434-6621 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Background Risk-adjusted treatment has led to outstanding improvements of the remission and survival rates of childhood acute lymphoblastic leukemia (ALL). Nevertheless, overtreatment-related toxicity and resistance to therapy have not been fully prevented. In the present study, we evaluated for the first time the clinical impact of the apoptosis-related BCL2L12 gene in prognosis and risk stratification of BFM-treated childhood ALL. Methods Bone marrow specimens were obtained from childhood ALL patients upon disease diagnosis and the end-of-induction (EoI; day 33) of the BFM protocol, as well as from control children. Following total RNA extraction and reverse transcription, BCL2L12 expression levels were determined by qPCR. Patients' cytogenetics, immunophenotyping and minimal residual disease (MRD) evaluation were performed according to the international guidelines. Results BCL2L12 expression was significantly increased in childhood ALL and correlated with higher BCL2/BAX expression ratio and favorable disease markers. More importantly, BCL2L12 expression was associated with disease remission, while the reduced BCL2L12 expression was able to predict patients' poor response to BFM therapy, in terms of M2-M3 response and MRD&amp;gt;/=0.1% on day 15. The survival analysis confirmed the significantly higher risk of the BFM-treated patients underexpressing BCL2L12 at disease diagnosis for early relapse and worse survival. Lastly, evaluation of BCL2L12 expression clearly strengthened the prognostic value of the established disease prognostic markers, leading to superior prediction of patients' outcome and improved specificity of BFM risk stratification. Conclusions The expression levels of the apoptosis-related BCL2L12 predict response to treatment and survival outcome of childhood ALL patients receiving BFM chemotherapy.</style></abstract><accession-num><style face="normal" font="default" size="100%">30016275</style></accession-num><notes><style face="normal" font="default" size="100%">Avgeris, MargaritisStamati, LampriniKontos, Christos KPiatopoulou, DespinaMarmarinos, AntoniosXagorari, MarietaBaka, MargaritaDoganis, DimitriosAnastasiou, TheodoraKosmidis, HelenGourgiotis, DimitriosScorilas, AndreasengGermany2018/07/18 06:00Clin Chem Lab Med. 2018 Nov 27;56(12):2104-2118. doi: 10.1515/cclm-2018-0507.</style></notes><auth-address><style face="normal" font="default" size="100%">Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece.Laboratory of Clinical Biochemistry - Molecular Diagnostics, Second Department of Pediatrics, National and Kapodistrian University of Athens, Medical School, &quot;P. &amp; A. Kyriakou&quot; Children's Hospital, Athens, Greece.Department of Pediatric Oncology, &quot;P. &amp; A. Kyriakou&quot; Children's Hospital, Athens, Greece.Laboratory of Hematology, &quot;P. &amp; A. Kyriakou&quot; Children's Hospital, Athens, Greece.</style></auth-address></record></records></xml>