<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Stamati, L.</style></author><author><style face="normal" font="default" size="100%">Avgeris, M.</style></author><author><style face="normal" font="default" size="100%">Kosmidis, H.</style></author><author><style face="normal" font="default" size="100%">Baka, M.</style></author><author><style face="normal" font="default" size="100%">Anastasiou, T.</style></author><author><style face="normal" font="default" size="100%">Piatopoulou, D.</style></author><author><style face="normal" font="default" size="100%">Scorilas, A.</style></author><author><style face="normal" font="default" size="100%">Gourgiotis, D.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Overexpression of BCL2 and BAX following BFM induction therapy predicts ch-ALL patients' poor response to treatment and short-term relapse</style></title><secondary-title><style face="normal" font="default" size="100%">J Cancer Res Clin Oncol</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Adolescent</style></keyword><keyword><style  face="normal" font="default" size="100%">Antineoplastic Combined Chemotherapy Protocols/*therapeutic use</style></keyword><keyword><style  face="normal" font="default" size="100%">apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Asparaginase/therapeutic use</style></keyword><keyword><style  face="normal" font="default" size="100%">bcl-2-Associated X Protein/biosynthesis/*metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Berlin-Frankfurt-Munster treatment protocol</style></keyword><keyword><style  face="normal" font="default" size="100%">Biomarkers, Tumor/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Bone Marrow Cells/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Bone Marrow/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Chemotherapy resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">Child</style></keyword><keyword><style  face="normal" font="default" size="100%">Child, Preschool</style></keyword><keyword><style  face="normal" font="default" size="100%">Childhood acute lymphoblastic leukemia (ch-ALL)</style></keyword><keyword><style  face="normal" font="default" size="100%">Daunorubicin/therapeutic use</style></keyword><keyword><style  face="normal" font="default" size="100%">Disease-Free Survival</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Induction Chemotherapy/methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Infant</style></keyword><keyword><style  face="normal" font="default" size="100%">Male</style></keyword><keyword><style  face="normal" font="default" size="100%">molecular tumor markers</style></keyword><keyword><style  face="normal" font="default" size="100%">Precursor Cell Lymphoblastic Leukemia-Lymphoma/*drug therapy/genetics/mortality</style></keyword><keyword><style  face="normal" font="default" size="100%">Prednisone/therapeutic use</style></keyword><keyword><style  face="normal" font="default" size="100%">Proto-Oncogene Proteins c-bcl-2/biosynthesis/*metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Treatment Outcome</style></keyword><keyword><style  face="normal" font="default" size="100%">Up-Regulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Vincristine/therapeutic use</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2015</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Nov</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">11</style></number><volume><style face="normal" font="default" size="100%">141</style></volume><pages><style face="normal" font="default" size="100%">2023-36</style></pages><isbn><style face="normal" font="default" size="100%">1432-1335 (Electronic)0171-5216 (Print)0171-5216 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">PURPOSE: The identification of childhood acute lymphoblastic leukemia (ch-ALL) patients who are at a higher risk of chemotherapy resistance and relapse is essential for successful treatment decisions, despite the application of novel therapies. The aim of the study is the evaluation of BCL2 and BAX expression for the prognosis of ch-ALL patients treated with Berlin-Frankfurt-Munster (BFM) backbone protocol. METHODS: Bone marrow specimens were obtained at the time of diagnosis and on day 33 following BFM treatment induction from 82 ch-ALL patients, as well as from 63 healthy children. Following extraction, total RNA was reverse transcribed and BCL2 and BAX expression levels were determined by qPCR. RESULTS: BCL2 expression and BCL2/BAX ratio were strongly upregulated in ch-ALL compared to healthy children and were correlated with favorable prognostic disease features. Increased levels of BCL2 and BAX expression were associated with disease remission, as ch-ALL patients with lower expression ran a significantly higher risk of M2-M3 response, positive MRD and poor survival outcome. Moreover, the upregulation of BCL2 and BAX following BFM treatment induction was shown to represent an independent predictor of patients' short-term relapse, which was further confirmed in ch-ALL patients with favorable prognostic markers. CONCLUSIONS: In conclusion, BCL2 and BAX could be effectively used for an enhanced prediction of BFM-treated patients' outcome.</style></abstract><accession-num><style face="normal" font="default" size="100%">25982455</style></accession-num><notes><style face="normal" font="default" size="100%">Stamati, LampriniAvgeris, MargaritisKosmidis, HelenBaka, MargaritaAnastasiou, TheodoraPiatopoulou, DespinaScorilas, AndreasGourgiotis, DimitriosengGermany2015/05/20 06:00J Cancer Res Clin Oncol. 2015 Nov;141(11):2023-36. doi: 10.1007/s00432-015-1982-6. Epub 2015 May 16.</style></notes><custom2><style face="normal" font="default" size="100%">PMC11824082</style></custom2><auth-address><style face="normal" font="default" size="100%">Laboratory of Clinical Biochemistry - Molecular Diagnostics, Second Department of Pediatrics, University of Athens Medical School, &quot;P&amp;A Kyriakou&quot; Children's Hospital, Levadias 13 Str., 115 27, Athens, Greece.Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, Panepistimiopolis, 157 01, Athens, Greece.Department of Pediatric Oncology, &quot;P&amp;A Kyriakou&quot; Children's Hospital, Thivon &amp; Levadias Str., 115 27, Athens, Greece.Laboratory of Hematology, &quot;P&amp;A Kyriakou&quot; Children's Hospital, Thivon &amp; Levadias Str., 115 27, Athens, Greece.Laboratory of Clinical Biochemistry - Molecular Diagnostics, Second Department of Pediatrics, University of Athens Medical School, &quot;P&amp;A Kyriakou&quot; Children's Hospital, Levadias 13 Str., 115 27, Athens, Greece. dgourg@med.uoa.gr.</style></auth-address></record></records></xml>