@article {7833,
	title = {Association between transcriptional activity, local chromatin structure, and the efficiencies of both subpathways of nucleotide excision repair of melphalan adducts},
	journal = {Cancer Research},
	volume = {69},
	year = {2009},
	note = {Cited By :12Export Date: 21 February 2017},
	month = {2009},
	pages = {4424 - 4433},
	abstract = {The repair of melphalan-induced N-alkylpurine monoadducts and interstrand cross-links was examined in different repair backgrounds, focusing on four genes (β-actin, p53, N-ras, and δ-globin) with dissimilar transcription activities. Adducts were found to be substrates for both global genome repair (GGR) and transcription-coupled repair (TCR), with TCR being less efficient than GGR. In nucleotide excision repair-deficient cells, adducts accumulated to similar levels in all four genes. The repair efficiency in different gene loci varied in a qualitatively and quantitatively similar way in both GGR-deficient and TCR-deficient backgrounds and correlated with transcriptional activity and local chromatin condensation. No strand-specific repair was found in GGR +/TCR+ cells, implying that GGR dominated. Adducts were lost over two sharply demarcated phases: a rapid phase resulting in the removal within 1 hour of up to \~{}80\% of the adducts, and a subsequent phase with t1/2 \~{}36 to 48 hours. Following pretreatment of cells with α-amanitin, the rate of transcription, the state of chromatin condensation, and the repair efficiencies (both TCR and GGR) of the transcribed β-actin, p53, and N-ras genes became similar to those of the nontranscribed δ-globin gene. In conclusion, a continuous, parallel variation of the state of transcription and local chromatin condensation, on one hand, and the rates of both GGR and TCR, on the other hand, have been shown. {\textcopyright}2009 American Association for Cancer Research.},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-66249147593\&doi=10.1158\%2f0008-5472.CAN-08-3489\&partnerID=40\&md5=aa401bf07fa514cf02685ea0d0c7610c},
	author = {Episkopou, H. and Kyrtopoulos, S.A. and Sfikakis, P.P. and Fousteri, M. and Dimopoulos, M.A. and Mullenders, L.H.F. and Souliotis, V.L.}
}
