@article {7842,
	title = {Expression and prognostic significance of kallikrein-related peptidase 8 protein levels in advanced ovarian cancer by using automated quantitative analysis},
	journal = {Thrombosis and Haemostasis},
	volume = {101},
	year = {2009},
	note = {Cited By :16Export Date: 21 February 2017},
	month = {2009},
	pages = {541 - 546},
	abstract = {Kallikrein-related peptidases, a subgroup of the serine protease enzyme family, are considered to be important prognostic biomarkers in cancer. In this study we sought to determine the prognostic value of kallikrein-related peptidase 8 (KLK8,hK8,KLK-8) in ovarian cancer using a novel method of compartmentalised in situ protein analysis.A tissue array composed of 150 advanced stage ovarian cancers, uniformly treated with surgical debulking followed by platinum-paclitaxel combination chemotherapy,was constructed. For the evaluation of kallikrein-related peptidase 8 protein expression, we used an immunofluorescence-based method of automated in situ quantitative protein analysis (AQUA). Mean follow-up time of the cohort was 34.35 months. One hundred twenty-six of 150 cases had sufficient tissue for AQUA analysis.There were significant correlations between tumour mask KLK8 protein expression levels and clinicopathological variables, including grade (p=0.0011), residual disease (p=0.0063) and clinical response to chemotherapy(p=0.0346). In univariate survival analysis there was a significant correlation between KLK8 tumour mask expression and five years progression-free survival, meanwhile it was not associated with five-year overall survival (p =0.0694). Specifically, low KLK8 expression correlated with better outcome (top vs. bottom quartile, p=0.0319). In multivariate survival analysis, adjusting for well-characterised prognostic variables, tumour KLK8 expression level retained its prognostic significance for progression-free survival (95\%Cl: 0.341-1.027, p=0.045). The possibility that KLK8 may be a suitable candidate as a diagnostic and prognostic marker warrants further investigation. {\textcopyright} 2009 Schattauer GmbH.},
	keywords = {Advanced ovarian cancer, AQUA, Kallikrein-related peptidase 8, Prognosis},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-62549103358\&doi=10.1160\%2fTH08-01-0052\&partnerID=40\&md5=999265a01129d52481c3b42b5d53a84c},
	author = {Kountourakis, P. and Psyrri, A. and Scorilas, A. and Markakis, S. and Kowaiski, D. and Camp, R.L. and Diamandis, E.P. and Dimopoulos, M.A.}
}
