@article {7988,
	title = {Subcellular localization and protein levels of cyclin-dependent kinase inhibitor p27 independently predict for survival in epithelial ovarian cancer},
	journal = {Clinical Cancer Research},
	volume = {11},
	year = {2005},
	note = {Cited By :28Export Date: 21 February 2017},
	month = {2005},
	pages = {8384 - 8390},
	abstract = {Purpose: p27 protein is regarded as a valuable prognostic biomarker in cancer with a potential use as a molecular target. However, different methods of immunohistochemical assessment have yielded conflicting results. Here, we sought to determine the prognostic value of p27 in ovarian cancer using a novel method of compartmentalized in situ protein analysis. Experimental Design: A tissue array composed of 150 advanced stage ovarian cancers uniformly treated, with surgical debulking followed by platinum-paclitaxel combination chemotherapy, was constructed. For evaluation of p27 protein expression, we used an immunofluorescence-based method of automated in situ quantitative measurement of protein analysis [automated quantitative analysis (AQUA)]. Results: The mean follow-up time of the patients was 34.3 months. Patients with low F{\'e}d{\'e}ration Internationale des Gynaecologistes et Obstetristes stage were more likely to have low nuclear p27 expression (P = 0.008). Low nuclear p27 expression was associated with improved 3-year overall survival (66\% versus 20\%, P = 0.0047) and disease-free survival (27\% versus 12\%, P = 0.022). In multivariable analysis, adjusting for well-characterized prognostic variables, low nuclear p27 expression level was the most significant prognostic factor for both disease-free and overall survival. Conclusions: Our results indicate that quantitative assessment of nuclear p27 expression level by automated in situ quantitative analysis is a strong predictor for outcome in ovarian cancer. {\textcopyright} 2005 American Association for Cancer Research.},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-28544433273\&doi=10.1158\%2f1078-0432.CCR-05-1270\&partnerID=40\&md5=423128fde42da221953f9140fb24a0e8},
	author = {Psyrri, A. and Bamias, A. and Yu, Z. and Weinberger, P.M. and Kassar, M. and Markakis, S. and Kowalski, D. and Efstathiou, E. and Camp, R.L. and Rimm, D.L. and Dimopoulos, M.A.}
}
