@article {8152,
	title = {The prevalence of bcl-2, p53, and Ki-67 immunoreactivity in transitional cell bladder carcinomas and their clinicopathologic correlates},
	journal = {Human Pathology},
	volume = {29},
	year = {1998},
	note = {Cited By :112Export Date: 21 February 2017},
	month = {1998},
	pages = {146 - 154},
	abstract = {The aim of this study was to investigate the expression of bcl-2, p53 oncoproteins, and Ki-67 antigen in a series of transitional cell bladder carcinomas and its relation to the traditional prognostic indicators and patient{\textquoteright}s survival. One hundred six cases with transitional cell carcinoma (TCC) were examined for detection of bcl-2, p53 proteins, and Ki-67 antigen (MIB1 antibody). Bcl-2 immunohisto-chemical positivity was observed in 52\% of TCCs and in 577\% of low-grade and 44\% of high-grade TCCs. Bcl-2 was also detected in normal urothelium and dysplastic lesions with basal cell expression, showed a significant inverse correlation with overall bcl-2 positivity. The loss of bcl-2 protein expression in higher-stage TCCs was statistically significant (pt = .01). P53 protein was overexpressed in 50\% of TCCs and more frequently in invasive and in carcinomas in situ than in superficial TCCs (Pt = .03). In contrast, detection of p53 was not observed in normal and dysplastic urothelium. P53 positivity was related to the degree of differentiation and to the stage of the disease (Pf = .01 and Pt = .03, respectively). Concerning Ki-67 antigen, its expression was found in 57.5\% of TCCs. There was a strong overall correlation of Ki-67 with tumor stage (Pt = .002) and grade (Pf = .002). Univariate statistical analysis showed that the expression of p53 and Ki-67 was significantly correlated to poor prognosis (p = .02, P = .02, respectively). On multivariate analysis, none of these markers but only stage and grade were significantly correlated to prognosis (P = .02, respectively). These findings suggest that overexpression of bcl-2 protein may be an early event in tumorigenesis. Tumors with loss of bcl-2 positivity and overexpression of p53 and Ki-67 had an unfavorable prognosis; however, in multivariate analysis, they had no independent prognostic value.},
	keywords = {Bcl-2, Ki-67, p53, transitional cell carcinoma},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031884935\&doi=10.1016\%2fS0046-8177\%2898\%2990225-8\&partnerID=40\&md5=d520b465f1c0b5e1de72bac0f33f51eb},
	author = {Nakopoulou, L. and Vourlakou, C. and Zervas, A. and Tzonou, A. and Gakiopoulou, H and Dimopoulos, M.-A.}
}
