@article {8165,
	title = {Long-range amplification of genomic DNA detects the t(2;5)(p23;q35) in anaplastic large-cell lymphoma, but not in other non-Hodgkin{\textquoteright}s lymphomas, Hodgkin{\textquoteright}s disease, or lymphomatoid papulosis},
	journal = {Annals of Oncology},
	volume = {8},
	year = {1997},
	note = {Cited By :17Export Date: 21 February 2017},
	month = {1997},
	pages = {S59 - S63},
	abstract = {Design: Determine the frequency of t(2;5)(p23;q35) in anaplastic large- cell lymphoma (ALCL), non-Hodgkin{\textquoteright}s lymphoma (NHL), Hodgkin{\textquoteright}s disease (HD), and lymphomatoid papulosis (LP). Patients and methods: The t(2;5) was detected with a long-range nested polymerase chain reaction (PCR) using 0,5 μg of DNA (60000-80000 cells), 5{\textquoteright}-primers from the NPM gene, 3{\textquoteright}primers from the ALK gene, agarose electrophoresis, hybridization, and autoradiography. Patients were evaluable if a 3016 base pair amplicon could be generated from tumor DNA with β-globin primers. Results: Amplicons were detected by PCR of genomic DNA from three ALCL cell lines and four primary ALCLs known to t(2;5) positive. DNA from t(2;5)-positive cell lines diluted 104-fold or 105-fold generated amplicons in 100\% or 20\% of reactions, respectively. Archival tumor DNA from 144 patients was amplifiable by β-globin amplicons in 126 (88\%) who are considered evaluable for this study. Twenty-two had ALCL, 69 other NHLs, 30 HD, and five LP. Genomic DNA PCR detected the t(2;5) in 5 of 22 with ALCL (23\%, 95\% confidence intervals [95\% CI] 8\%-45\%) but not in those with NHLs, HD, or LP. Among ALCLs the t(2;5) was confined to 5 of 20 with nodal presentations (25\%, 95\% CI 9\%-49\%), among whom it was seen in 5 of 15 with T- cell or null-cell phenotype (33\%, 95\% CI 12\%62\%), in 4 of 11 with age \&lt; 40 years (36\%, 95\% CI 11\%-69\%), and in 4 of 9 with nodal presentations, T-cell or null-cell phenotype, and age \&lt;40 years (44\%, 95\% CI 14\%-79\%). Amplicon sizes were different between cell lines and patients, reflecting unique genomic DNA breakpoints, as confirmed by DNA sequencing, and served as an internal control against specimen cross-contamination in the laboratory. Conclusions: Long-range PCR of genomic DNA detects t(2;5) only in ALCL but not in other NHLs, HD, or LP. Long-range PCR may be useful in establishing diagnosis, determining prognosis, and monitoring minimal residual disease in ALCL.},
	keywords = {Anaplastic large-cell lymphoma, Genomic DNA, Long-range PCR, T(2;5)(p23;q35)},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-8544278124\&partnerID=40\&md5=17baa74007802ec68e254c7d5e0d81bb},
	author = {A.H. Sarris and Luthra, R. and Papadimitracopoulou, V. and Waasdorp, M. and Dimopoulos, M.A. and McBride, J.A. and Cabanillas, F. and Duvic, M. and Deisseroth, A. and Morris, S.W. and Pugh, W.C.}
}
