<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bamias, A.</style></author><author><style face="normal" font="default" size="100%">Tsiatas, M. L.</style></author><author><style face="normal" font="default" size="100%">Kafantari, E.</style></author><author><style face="normal" font="default" size="100%">Liakou, C.</style></author><author><style face="normal" font="default" size="100%">Rodolakis, A.</style></author><author><style face="normal" font="default" size="100%">Voulgaris, Z.</style></author><author><style face="normal" font="default" size="100%">Vlahos, G.</style></author><author><style face="normal" font="default" size="100%">Papageorgiou, T.</style></author><author><style face="normal" font="default" size="100%">Tsitsilonis, O.</style></author><author><style face="normal" font="default" size="100%">Bamia, C.</style></author><author><style face="normal" font="default" size="100%">Papatheodoridis, G.</style></author><author><style face="normal" font="default" size="100%">Politi, Ek.</style></author><author><style face="normal" font="default" size="100%">Archimandritis, A</style></author><author><style face="normal" font="default" size="100%">Antsaklis, A.</style></author><author><style face="normal" font="default" size="100%">Dimopoulos, M.A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Significant differences of lymphocytes isolated from ascites of patients with ovarian cancer compared to blood and tumor lymphocytes. Association of CD3+CD56+ cells with platinum resistance</style></title><secondary-title><style face="normal" font="default" size="100%">Gynecologic Oncology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Immune response</style></keyword><keyword><style  face="normal" font="default" size="100%">NK-T</style></keyword><keyword><style  face="normal" font="default" size="100%">Ovarian cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">Tregs</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2007</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250159834&amp;doi=10.1016%2fj.ygyno.2007.02.029&amp;partnerID=40&amp;md5=0640ce45285f5a1530046431cb5eb6de</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">106</style></volume><pages><style face="normal" font="default" size="100%">75 - 81</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Objectives: Tumor infiltrating lymphocytes (TILs) and T regulatory cells (Tregs) have been associated with prognosis in ovarian cancer, but their prognostic significance in ascites has not been studied. We performed a prospective study of T lymphocytes isolated from ascites from patients with ovarian carcinoma and we compared them with the respective populations in blood and tumors. Methods: Mononuclear cells from ascites (n = 71) and blood were isolated by Ficoll, while tumor lymphocytes (n = 20) were obtained upon mechanical dissociation. Phenotypic analysis was performed with flow cytometry. Ascites from 10 patients with cirrhosis was used as control. Results: Tregs containing CD4+CD25+ cells, NK-T containing CD3+CD56+ cells and CD69 and HLADR expression of CD4 and CD8 lymphocytes were significantly increased in tumor ascites compared to blood and control ascites. A selective accumulation of these populations in the ascites of cancer patients, was suggested by the significantly higher ascites/blood (A/B) ratios in cancer patients but not controls. Cancer cell content in ascites was correlated with CD4+CD25+, CD4+CD69+, CD4+HLADR+ and CD8+CD69+ cells. There was no correlation of lymphocyte populations between ascites and samples from peritoneal metastases. Higher tumor grade was associated with increased A/B CD4+CD25+ ratio and reduced CD3+CD56+ cells, while platinum resistance was associated with reduced A/B CD3+CD56+ ratio. Conclusions: There are significant differences of CD3+CD56+ and CD25+CD4+ lymphocytes and increase in lymphocyte activation between blood, ascites and peritoneal metastases from patients with ovarian cancer. The selective accumulation of CD3+CD56+ population in ascites may be a predictive factor for platinum resistance. © 2007 Elsevier Inc. All rights reserved.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><notes><style face="normal" font="default" size="100%">Cited By :38Export Date: 21 February 2017</style></notes></record></records></xml>