<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">A.H. Sarris</style></author><author><style face="normal" font="default" size="100%">Luthra, R.</style></author><author><style face="normal" font="default" size="100%">Papadimitracopoulou, V.</style></author><author><style face="normal" font="default" size="100%">Waasdorp, M.</style></author><author><style face="normal" font="default" size="100%">Dimopoulos, M.A.</style></author><author><style face="normal" font="default" size="100%">McBride, J.A.</style></author><author><style face="normal" font="default" size="100%">Cabanillas, F.</style></author><author><style face="normal" font="default" size="100%">Duvic, M.</style></author><author><style face="normal" font="default" size="100%">Deisseroth, A.</style></author><author><style face="normal" font="default" size="100%">Morris, S.W.</style></author><author><style face="normal" font="default" size="100%">Pugh, W.C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Long-range amplification of genomic DNA detects the t(2;5)(p23;q35) in anaplastic large-cell lymphoma, but not in other non-Hodgkin's lymphomas, Hodgkin's disease, or lymphomatoid papulosis</style></title><secondary-title><style face="normal" font="default" size="100%">Annals of Oncology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anaplastic large-cell lymphoma</style></keyword><keyword><style  face="normal" font="default" size="100%">Genomic DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Long-range PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">T(2;5)(p23;q35)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1997</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1997</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.scopus.com/inward/record.uri?eid=2-s2.0-8544278124&amp;partnerID=40&amp;md5=17baa74007802ec68e254c7d5e0d81bb</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">S59 - S63</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Design: Determine the frequency of t(2;5)(p23;q35) in anaplastic large- cell lymphoma (ALCL), non-Hodgkin's lymphoma (NHL), Hodgkin's disease (HD), and lymphomatoid papulosis (LP). Patients and methods: The t(2;5) was detected with a long-range nested polymerase chain reaction (PCR) using 0,5 μg of DNA (60000-80000 cells), 5'-primers from the NPM gene, 3'primers from the ALK gene, agarose electrophoresis, hybridization, and autoradiography. Patients were evaluable if a 3016 base pair amplicon could be generated from tumor DNA with β-globin primers. Results: Amplicons were detected by PCR of genomic DNA from three ALCL cell lines and four primary ALCLs known to t(2;5) positive. DNA from t(2;5)-positive cell lines diluted 104-fold or 105-fold generated amplicons in 100% or 20% of reactions, respectively. Archival tumor DNA from 144 patients was amplifiable by β-globin amplicons in 126 (88%) who are considered evaluable for this study. Twenty-two had ALCL, 69 other NHLs, 30 HD, and five LP. Genomic DNA PCR detected the t(2;5) in 5 of 22 with ALCL (23%, 95% confidence intervals [95% CI] 8%-45%) but not in those with NHLs, HD, or LP. Among ALCLs the t(2;5) was confined to 5 of 20 with nodal presentations (25%, 95% CI 9%-49%), among whom it was seen in 5 of 15 with T- cell or null-cell phenotype (33%, 95% CI 12%62%), in 4 of 11 with age &amp;lt; 40 years (36%, 95% CI 11%-69%), and in 4 of 9 with nodal presentations, T-cell or null-cell phenotype, and age &amp;lt;40 years (44%, 95% CI 14%-79%). Amplicon sizes were different between cell lines and patients, reflecting unique genomic DNA breakpoints, as confirmed by DNA sequencing, and served as an internal control against specimen cross-contamination in the laboratory. Conclusions: Long-range PCR of genomic DNA detects t(2;5) only in ALCL but not in other NHLs, HD, or LP. Long-range PCR may be useful in establishing diagnosis, determining prognosis, and monitoring minimal residual disease in ALCL.</style></abstract><issue><style face="normal" font="default" size="100%">SUPPL. 2</style></issue><notes><style face="normal" font="default" size="100%">Cited By :17Export Date: 21 February 2017</style></notes></record></records></xml>