@article {29995,
	title = {A modified protocol for isolation and purity evaluation of a staphylococcal acidic polysaccharide by chromatography and capillary electrophoresis},
	journal = {Biomed ChromatogrBiomed Chromatogr},
	volume = {25},
	number = {5},
	year = {2011},
	note = {Krevvata, Maria IAfratis, NikosSpiliopoulou, AnastasiaMalavaki, Christina JKolonitsiou, FevroniaAnastassiou, EvangelosKaramanos, Nikos KengEngland2010/08/25Biomed Chromatogr. 2011 May;25(5):531-4. doi: 10.1002/bmc.1490. Epub 2010 Aug 23.},
	month = {May},
	pages = {531-4},
	edition = {20100823},
	abstract = {The extracellular slime of Staphylococcus epidermidis contains, amongst various macromolecules, an acidic polysaccharide (PS) of a molecular mass of 20 kDa with significant antigenic and biological properties. The isolation procedure used so far includes multiple fractionations in anion-exchange chromatographic columns before its final purification by gel filtration chromatography. This protocol is laborious, time-consuming and includes the risk of unnecessary loss of PS quantities. Because of the significance of this PS, a modified protocol resulting in an easier and quicker isolation procedure was developed. Furthermore, identification, purity, charge density and molecular integrity of the isolated polysaccharide were evaluated by a reverse-polarity capillary electrophoresis method.},
	keywords = {Electrophoresis, Capillary/*methods, Formates/chemistry, Hydrogen-Ion Concentration, Polysaccharides, Bacterial/chemistry/*isolation \& purification, Staphylococcus epidermidis/*chemistry},
	isbn = {1099-0801 (Electronic)0269-3879 (Linking)},
	author = {Krevvata, M. I. and Afratis, N. and Spiliopoulou, A. and Malavaki, C. J. and Kolonitsiou, F. and Anastassiou, E. and Karamanos, N. K.}
}
