<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kouvidi, K.</style></author><author><style face="normal" font="default" size="100%">Berdiaki, A.</style></author><author><style face="normal" font="default" size="100%">Nikitovic, D.</style></author><author><style face="normal" font="default" size="100%">Katonis, P.</style></author><author><style face="normal" font="default" size="100%">Afratis, N.</style></author><author><style face="normal" font="default" size="100%">Hascall, V. C.</style></author><author><style face="normal" font="default" size="100%">Karamanos, N. K.</style></author><author><style face="normal" font="default" size="100%">Tzanakakis, G. N.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Role of receptor for hyaluronic acid-mediated motility (RHAMM) in low molecular weight hyaluronan (LMWHA)-mediated fibrosarcoma cell adhesion</style></title><secondary-title><style face="normal" font="default" size="100%">J Biol ChemJ Biol Chem</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*Gene Expression Regulation, Neoplastic</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Adhesion</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Line, Tumor</style></keyword><keyword><style  face="normal" font="default" size="100%">Extracellular Matrix Proteins/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Fibrosarcoma/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Focal Adhesion Protein-Tyrosine Kinases/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Hyaluronan Receptors/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Hyaluronic Acid/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitogen-Activated Protein Kinase 1/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitogen-Activated Protein Kinase 3/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Weight</style></keyword><keyword><style  face="normal" font="default" size="100%">Oligosaccharides/chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">RNA, Small Interfering/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">signal transduction</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Nov 4</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">44</style></number><edition><style face="normal" font="default" size="100%">20110913</style></edition><volume><style face="normal" font="default" size="100%">286</style></volume><pages><style face="normal" font="default" size="100%">38509-38520</style></pages><isbn><style face="normal" font="default" size="100%">1083-351X (Electronic)0021-9258 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Hyaluronan (HA) modulates key cancer cell functions through interaction with its CD44 and receptor for hyaluronic acid-mediated motility (RHAMM) receptors. HA was recently found to regulate the migration of fibrosarcoma cells in a manner specifically dependent on its size. Here, we investigated the effect of HA/RHAMM signaling on the ability of HT1080 fibrosarcoma cells to adhere onto fibronectin. Low molecular weight HA (LMWHA) significantly increased (p &lt;/= 0.01) the adhesion capacity of HT1080 cells, which high molecular weight HA inhibited. The ability of HT1080 RHAMM-deficient cells, but not of CD44-deficient ones, to adhere was significantly decreased (p &lt;/= 0.001) as compared with control cells. Importantly, the effect of LMWHA on HT1080 cell adhesion was completely attenuated in RHAMM-deficient cells. In contrast, adhesion of RHAMM-deficient cells was not sensitive to high molecular weight HA treatment, which identifies RHAMM as a specific conduit of the LMWHA effect. Western blot and real time-PCR analyses indicated that LMWHA significantly increased RHAMM transcript (p &lt;/= 0.05) and protein isoform levels (53%, 95 kDa; 37%, 73 kDa) in fibrosarcoma cells. Moreover, Western blot analyses showed that LMWHA in a RHAMM-dependent manner enhanced basal and adhesion-dependent ERK1/2 and focal adhesion kinase (FAK) phosphorylation in HT1080 cells. Utilization of a specific ERK1/2 inhibitor completely inhibited (p &lt;/= 0.001) LMWHA-dependent adhesion, suggesting that ERK1/2 is a downstream effector of LMWHA/RHAMM signaling. Likewise, the utilization of the specific ERK1 inhibitor resulted in a strong down-regulation of FAK activation in HT1080 cells, which identifies ERK1/2 as a FAK upstream activator. In conclusion, our results suggest that RHAMM/HA interaction regulates fibrosarcoma cell adhesion via the activation of FAK and ERK1/2 signaling pathways.</style></abstract><accession-num><style face="normal" font="default" size="100%">21914806</style></accession-num><notes><style face="normal" font="default" size="100%">Kouvidi, KaterinaBerdiaki, AikateriniNikitovic, DraganaKatonis, PavlosAfratis, NikosHascall, Vincent CKaramanos, Nikos KTzanakakis, George Neng2011/09/15J Biol Chem. 2011 Nov 4;286(44):38509-38520. doi: 10.1074/jbc.M111.275875. Epub 2011 Sep 13.</style></notes><custom2><style face="normal" font="default" size="100%">PMC3207394</style></custom2><auth-address><style face="normal" font="default" size="100%">Department of Histology-Embryology, University of Crete, Heraklion 71003, Greece.Department of Orthopaedics, Medical School, University of Crete, Heraklion 71003, Greece.Laboratory of Biochemistry, Department of Chemistry, University of Patras, Patras 26110, Greece.Cleveland Clinic, Biomedical Engineering ND-20, Cleveland, Ohio 44195.Department of Histology-Embryology, University of Crete, Heraklion 71003, Greece. Electronic address: tzanakak@med.uoc.gr.</style></auth-address><remote-database-name><style face="normal" font="default" size="100%">Medline</style></remote-database-name><remote-database-provider><style face="normal" font="default" size="100%">NLM</style></remote-database-provider></record></records></xml>